MDR Acinetobacter Medium is a selective and differential medium, containing some common antibiotics for which many multi-drug resistant (MDR) strains of Acinetobacter are resistant, intended for use as a plating technique to obtain a pure culture of MDR Acinetobacter.

The selective components in MDR Acinetobacter Medium comprise the selective components in Leeds Acinetobacter Medium, as well as agents that inhibit the growth of non-MDR strains of Acinetobacter spp. The selective components in Leeds Acinetobacter Medium are traditionally cefsulodin and cephradine, which inhibit the growth of non-Acinetobacter gram-negative bacteria, and vancomycin, which inhibits the growth of gram-positive bacteria. Acinetobacter Medium substitutes antibiotics for the some of the antibiotics in the original reference. The presence of fructose and sucrose and neutral red, a pH indicator, allows the differentiation of bacteria based on the products released when the bacteria use fructose and sucrose as a nutrient source. The colonies of those that release acidic products are yellow, while the colonies of those that release alkaline products are red.

MDR Acinetobacter Medium is available in a monoplate (G259). Acinetobacter Biplate is a two-section Petri dish (J135). One individual component of the biplate is Leeds Acinetobacter Medium. The other individual component of the biplate is MDR Acinetobacter Medium.

Acinetobacter spp. will produce light pink mucoid colonies with a pink to mauve color diffused into the medium. The colonies are circular, convex, smooth, and opaque with entire margins of 1 to 2 mm in diameter after 24 hours at 35 degrees C.