|Morphology:||Cocci. Tetrads are formed in liquid media.|
|Size:||1.0 - 2.0 micrometers in diameter.|
Growth on solid media is generally sparse. Colonies are small, semi-transparent, white or gray with a beaded appearance. Greening reaction is produced on blood agar presumably as a result of H 2 O 2 production.
Facultatively anaerobic. Chemoorganoheterotrophic with a respiratory type metabolism. Produces acid without gas from various carbohydrates.
KEY BIOCHEMICAL REACTIONS
- Catalase-negative or weak.
- Gelatin is not liquified.
- Salt tolerant; will grow in 6.5% NaCl.
- Bile tolerant; will grow in up to 40% bile.
- Grows at pH 9.6.
- Optimum temperature is 30 degrees C. Grows at 10 degrees C., but not at 45 degrees C.
Frequently isolated from two widely divergent sources: as a common airborne organism in hospital environments and as a marine organism causing a fatal disease of lobsters.
Opportunistic pathogen. Generally saprophytic but has been associated with human endocarditis. Causes a fatal disease in lobsters called gaffkemia. A. urinae , a new species, was recently implicated as a pathogen in patients predisposed to urinary tract infection.
|For culture:||Columbia Blood Agar, Blood Agar 5%, Tryptic Soy Agar, MRS Broth, or Brain Heart Infusion Broth (BHI).|
|For selective isolation:||MacConkey Agar or Baird-Parker Agar.|
|For maintenance:||Blood Agar 5%, Tryptic Soy Agar, or Brucella Broth with Glycerol.|
|Temperature:||35 degrees C.|
|Time:||18 - 24 hours.|
1. Holt, J.G., et al. 1994. Bergey's Manual of Determinative Bacteriology , 9th ed. Williams & Wilkins, Baltimore, MD.
2. Holt, J.G., et al. 1986. Bergey's Manual of Systemic Bacteriology , Volumes I & II. Williams & Wilkins, Baltimore, MD.
3. The Oxoid Vade-Mecum of Microbiology . 1993. Unipath Ltd., Basingstoke, UK.
4. Murray, P.R., et al. 1995. Manual of Clinical Microbiology , 6th ed. American Society for Microbiology, Washington, D.C.