BLOOD AGAR

Cat. no. A10 Blood Agar 5%, 15x100mm Plate, 19ml 10 plates/bag
Cat. no. A10BX Blood Agar 5%, 15x100mm Plate, 19ml 100 plates/box
Cat. no. A17BX Blood Agar 8%, 15x100mm Plate, 18ml 100 plates/box
Cat. no. A71 Blood Agar 10%, 15x100mm Plate, 18ml 10 plates/bag
Cat. no. A127 Blood Agar 5%, 86x128mm Omni Tray, 30ml 10 plates/bag
Cat. no. GA10 Blood Agar 5%, 15x100mm Plate, 19ml
(reduced stacking ring)
10 plates/bag
Cat. no. H28 Blood Agar 5%, 15x150mm Plate, 70ml 10 plates/bag
Cat. no. J22 Blood Agar 5% / Eosin Methylene Blue (EMB), Levine Agar, 15x100mm Biplate, 10ml/10ml 10 plates/bag
Cat. no. J32 Blood Agar 5% / MacConkey Agar, 15x100mm Biplate,
10ml/10ml
10 plates/bag
Cat. no. J32BX Blood Agar 5% / MacConkey Agar, 15x100mm Biplate,
10ml/10ml
100 plates/box
Cat. no. J41 Blood Agar 5% / Chocolate Agar with Bacitracin,
15x100mm Biplate, 10ml/10ml
10 plates/bag
Cat. no. J42 Blood Agar 5% / Chocolate Agar, 15x100mm Biplate,
10ml/10ml
10 plates/bag
Cat. no. J92 Blood Agar 5% / Group A Beta Strep Agar, 15x100mm Biplate, 10ml/10ml 10 plates/bag
Cat. no. J93 Blood Agar 5% / Blood Agar 5%, 15x100mm Biplate,
10ml/10ml
10 plates/bag
Cat. No. J119 Blood Agar 5% / HardyCHROM™ UTI, 15x100mm Biplate,
10ml/10ml
10 plates/bag
Cat. no. J160 Tryptic Soy Agar (TSA) / Blood Agar 5%, 15x100mm,
10ml/10ml
10 plates/bag
Cat. no. L12 Blood Agar 5%, 16x100mm Tube, 5.5ml Slant 20 tubes/box
Cat. no. P33 Blood Agar 5%, 15x60mm Contact Plate, 15ml 10 plates/bag

INTENDED USE

Hardy Diagnostics Blood Agar products are recommended for use as general purpose growth media for the isolation, cultivation, and differentiation of a wide variety of microorganisms.

Cat. no. P33 is not intended to be used for the diagnosis of human disease.

SUMMARY

Tryptic Soy Agar is the basal medium for the Blood Agar products. Sheep blood has been added, in various concentrations, to facilitate the growth of some organisms, and for the observation of hemolytic reactions. The absence of reducing sugars and carbohydrates allows the hemolysis to occur without hindrance.

Blood Agar 5% Contact Plate (Cat. no. P33), is recommended for use in the cultivation of microorganisms from environmental surfaces. The contact plate has a specified grid molded into the bottom of the plate. They are used primarily to monitor microbial contamination, for enumeration of microbial colonies growing on a variety of surfaces, and to assist in determining surface sanitation.

FORMULA

Ingredients per liter of deionized water:*

Pancreatic Digest of Casein 15.0gm
Peptic Digest of Soybean Meal 5.0gm
Sodium Chloride 5.0gm
Sheep Blood 50.0ml
Agar 12.0gm

Final pH 7.3 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

In addition, Blood Agar 8% (Cat. no. A17BX) contains 8.0% sheep blood and Blood Agar 10% (Cat. no. A71) contains 10.0% sheep blood.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), hemolysis, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

For Cat. nos. A10, A10BX, A17BX, A127, A71, GA10, H28, J22, J32, J32BX, J41, J42, J92, J93, J119, J160, and L12.

For Cat. no. P33

PROCEDURE

Specimen Collection: Infectious material should be submitted directly to the laboratory without delay and protected from excessive heat and cold. If there is to be a delay in processing, the specimen should be inoculated onto an appropriate transport media and refrigerated until inoculation. Consult listed references for information on specimen collection.(1-5)

Prepared media should be inoculated, incubated, and results recorded according to accepted procedures described in the listed reference texts.(1-5)

Refer to the document " Contact Plate Media " on the Hardy Diagnostics Technical Document website for more information regarding the method of use for Blood Agar 5% Contact Plate (Cat. no. P33).

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Streptococcus pneumoniae
ATCC® 6305
A 24hr 35ºC Aerobic or CO2** Growth; alpha-hemolysis
Streptococcus pyogenes
ATCC® 19615***
A 24hr 35ºC Aerobic or CO2** Growth; beta-hemolysis
Staphylococcus aureus
ATCC® 25923***
A 24hr 35ºC Aerobic or CO2** Growth; beta-hemolysis
Escherichia coli
ATCC® 25922
A 24hr 35ºC Aerobic or CO2** Growth
Enterococcus faecalis
ATCC® 29212
A 24hr 35ºC Aerobic or CO2** Growth
CAMP Test:
Staphylococcus aureus
ATCC® 33862
H 18-24hr 35ºC Aerobic Growth
Streptococcus agalactiae
ATCC® 12386
H 18-24hr 35ºC Aerobic Growth; positive (enhanced arrowhead hemolysis)
Streptococcus pyogenes
ATCC® 19615
H 18-24hr 35ºC Aerobic Growth; negative (no enhanced arrowhead hemolysis)

** Atmosphere of incubation is enriched with 5-10% CO2 for Cat. no. J92.

*** Recommended QC strains for User Quality Control according to the CLSI document M22 when applicable.

USER QUALITY CONTROL

PHYSICAL APPEARANCE

Blood Agar products should appear opaque, and cherry red in color.

S. pyogenes growing on Blood Agar

Streptococcus pyogenes (ATCC® 19615) colonies growing on Blood Agar, 5% Sheep (Cat. no. A10). Incubated aerobically for 24 hours at 35ºC.

S. aureus growing on Blood Agar

Staphylococcus aureus (ATCC® 25923) colonies growing on Blood Agar, 5% Sheep (Cat. no. A10). Incubated aerobically for 24 hours at 35ºC.



E. coli growing on Blood Agar

Escherichia coli (ATCC® 25922) colonies growing on Blood Agar, 5% Sheep (Cat. no. A10). Incubated aerobically for 24 hours at 35ºC.

S. pneumoniae growing on Blood Agar

Streptococcus pneumoniae (ATCC® 6305) colonies growing on Blood Agar, 5% Sheep (Cat. no. A10). Incubated in CO2 for 24 hours at 35ºC.



REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

5. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

ATCC is a registered trademark of the American Type Culture Collection.

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