BLOOD AGAR WITH AMPICILLIN

Cat. no. A12 Blood Agar with Ampicillin, 15x100mm Plate, 17ml 10 plates/bag

INTENDED USE

Hardy Diagnostics Blood Agar with Ampicillin is recommended for the cultivation and selective isolation of Aeromonas spp.

SUMMARY

Aeromonas hydrophila , an aquatic pathogen, has been associated with numerous clinical situations involving immunocompetent individuals who have presented with wound infections, septicemia, meningitis, osteomyelitis, pelvic abscesses, respiratory and ocular infections, and gastroenteritis. (6) The organism is an oxidase-positive, gram-negative bacillus closely related to Vibrio cholerae .

Aeromonas spp. grow readily on most laboratory media over a temperature range of 4 to 42ºC., with an optimum temperature of approximately 30ºC. (7,8) A large scale study conducted by Kelly et al. revealed Blood Agar with Ampicillin to be the single best medium for the isolation of aeromonads from stool specimens. (9)

Hardy Diagnostics Blood Agar with Ampicillin is composed of Tryptic Soy Agar with 5% sheep blood. Ampicillin is incorporated into the medium to inhibit most Enterobacteriaceae and some gram-positive bacteria.

FORMULA

Ingredients per liter of deionized water:*

Pancreatic Digest of Casein 15.0gm
Peptic Digest of Soybean Meal 5.0gm
Sodium Chloride 5.0gm
Ampicillin 10.0mg
Sheep Blood 50.0ml
Agar 15.4gm

Final pH 7.3 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), hemolysis, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

PROCEDURE

Specimen Collection: Infectious material should be submitted directly to the laboratory without delay and protected from excessive heat and cold. If there is to be a delay in processing, the specimen should be inoculated onto an appropriate transport media and refrigerated until inoculation. Consult listed references for information on specimen collection. (1-5)

Prepared media should be inoculated, incubated, and results recorded according to accepted procedures described in the listed reference texts. (1-5)

INTERPRETATION OF RESULTS

Aeromonas should appear as smooth, convex, grayish colonies 1-3mm in diameter; 24% may be non-hemolytic. (10)

LIMITATIONS

Blood Agar with Ampicillin is a selective growth medium and may inhibit the growth of certain species of the organism for which it is designed to isolate.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Aeromonas hydrophila
ATCC ® 7966
A 18-24hr 35°C Aerobic Growth
Escherichia coli
ATCC ® 25922
B 18-24hr 35°C Aerobic Partial to complete inhibition

User Quality Control

PHYSICAL APPEARANCE

Blood Agar with Ampicillin should appear opaque, and cherry red in color.

A. hydrophila growing on Blood Agar with Ampicillin

Aeromonas hydrophila (ATCC ® 7966) colonies growing on Blood Agar with Ampicillin (Cat. no. A12). Incubated aerobically for 24 hours at 35ºC.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Versalovic, J., et al. Manual of Clinical Microbiology. American Society for Microbiology, Washington, D.C.

3. Tille, P.M., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

5. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology. J.B. Lippincott Company, Philadelphia, PA.

6. Janda, J.M. and P.S. Duffey. 1988. Rev. Infect. Dis. ; 10:980-997.

7. George, W.L., MD. presented at the 1985 Microbiology Conference, sponsored by UCLA at Yosemite, CA.

8. Carnahan, A.M., MS. 1991. Clin. Micro. News ; Vol. 13, No. 22.

9. Kelly, M.T., et al. 1988. J. Clin. Microbiol. ; 26:1738-1740.

10. Millership, W.E., et al. 1983. J. Clin. Pathol. ; 36:920-923.


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