BLUEMOUNT™

Cat. no. Z137 BlueMount™ 15ml

INTENDED USE

Hardy Diagnostics BlueMount™ is recommended for the permanent mounting and staining of yeasts and molds.

SUMMARY

Hardy Diagnostics BlueMount™ is a formulation of lactophenol cotton blue with polyvinyl alcohol and is recommended for preparing permanent, long-lasting mounts of fungal wet preparations or slide cultures. BlueMount™ may be used as a replacement for lactophenol cotton blue. BlueMount™ consists of lactic acid, phenol, cotton blue, and polyvinyl alcohol (PVA). Lactic acid serves as a clearing agent and also helps preserve fungal structures. Phenol acts as a killing agent. Cotton blue is an acid dye that stains the chitin present in the cell walls of fungi. Polyvinyl alcohol (PVA) acts as a fixative, thus allowing the mount to be saved for years. (7) Once completely dry, the permanent mounts will not dissolve in ether, xylene, or alcohols. (7)

REAGENT FORMULA*

Phenol 440.0gm
Lactic Acid 440.0gm
Polyvinyl Alcohol 150.0gm
Cotton Blue 1.0gm
Deionized Water 1000.0ml

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-30ºC. Products should not be used if there are any signs of contamination, deterioration, or if the expiration date has passed. Product is light sensitive; protect from light.

PRECAUTIONS

This product is not recommended for use with MycoMount™ (Cat. no. MM40). Application of MycoMount™ to a BlueMount™ fungal preparation will deteriorate the MycoMount™ adhesive backing and result in obstruction of fungal structures upon microscopic observation.

Warning: This product causes irritation, may cause eye burns and is harmful if inhaled. Avoid contact with skin. Use with adequate ventilation. Wash thoroughly after handling.

Refer to the SDS Search instructions on the Hardy Diagnostics website for more information.

PROCEDURE

Specimen Collection: This product is intended to be used primarily with pure cultures, although certain specimens may be examined directly using this stain. Consult appropriate references for further information concerning the use of this product with specimens. (7)

Method of Use: Place one drop of BlueMount™ in the center of a clean slide. Remove a fragment of the fungus colony 2-3 mm from the colony edge using an inoculating or teasing needle. Place the fragment in the drop of stain and tease gently. Apply a cover slip. Do not push down or tap the cover slip as this may dislodge the conidia from the conidiophores. Take care to prevent coverslip runover or from dropping the stain on the bench top (once the solution dries, it is difficult to remove). Spills may be cleaned with water before it dries. The preparation can be examined immediately under low and high-dry magnification for the presence of characteristic mycelia and fruiting structures. Once the slide has dried on a flat surface for a period of two to four days, it can be examined under oil immersion, cleansed in xylene, or decontaminated by dipping in disinfectant. (7)

INTERPRETATION OF RESULTS

Consult appropriate references for diagnostic features of fungi isolated in clinical and non-clinical specimens. (1-8)

LIMITATIONS

BlueMount™ is useful in the recognition and presumptive identification of fungi. Additional characteristics including colony morphology and biochemical tests should be used where appropriate for definitive identification. For further information, consult the appropriate references. (1-8)

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, culture media, incinerators, incubators, pasteur pipets, slides, coverslips, microscopes, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Reaction
Aspergillus brasiliensis
formerly A. niger
ATCC ® 16404

Delicate blue hyphae and fruiting structures with a clear background
Trichophyton mentagrophytes
ATCC ® 9533
Delicate blue hyphae and fruiting structures with a clear background

User Quality Control

PHYSICAL APPEARANCE

BlueMount™ should appear slightly viscous, and deep blue in color.

BlueMount

BlueMount™

REFERENCES

1. Versalovic, J., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

4. "Cumitech 11; Practical Methods for Culture and Identification of Fungi in the Clinical Microbiology Laboratory". 1980. American Society for Microbiology, Washington D.C.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

6. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

7. Larone, D.H. Medically Important Fungi: A Guide to Identification, American Society for Microbiology. Washington, D.C.

8. Kwon-Chung, K.J. and J.E. Bennett. 1992. Medical Mycology. Lea and Febiger, Malvern, PA.


ATCC is a registered trademark of the American Type Culture Collection.

121415hh