BRILLIANT GREEN BILE BROTH WITH DURHAM TUBE
|Cat. no. K09||
Brilliant Green Bile Broth with
Durham Tube, 20x125mm
Tube with Screw Cap, 13ml
|Cat. no. K66||
Brilliant Green Bile Broth with Durham Tube, 16x125mm
Tube with Screw Cap, 10ml
Hardy Diagnostics Brilliant Green Bile Broth with Durham Tube is recommended for the detection of coliforms in water and other samples.
This product is not intended to be used for the diagnosis of human disease.
The formulation for Brilliant Green Bile Broth was developed by the Association of Official Analytical Communities (AOAC) and the American Public Health Association (APHA). (3-7) The basal medium is composed of peptone, which contains 2% bile, and brilliant green dye. Bile is inhibitory to gram-positive microorganisms, while brilliant green dye inhibits selected gram-negative bacilli. Lactose-fermenting organisms resistant to these inhibitors are detected by the production of gas. Gas production is noted by the appearance of bubbles in the durham tube.
Ingredients per liter of deionized water:*
|Pancreatic Digest of Gelatin||10.0gm|
Final pH 7.2 +/- 0.2 at 25ºC.
* Adjusted and/or supplemented as required to meet performance criteria.
STORAGE AND SHELF LIFE
Storage: Upon receipt, store media at 2-30ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture and freezing.
Specimen Collection: Consult listed references for information on specimen collection. (3-6) Infectious material should be submitted directly to the laboratory without delay and protected from excessive heat and cold. If there is to be a delay in processing, the specimen should be inoculated onto an appropriate transport medium and refrigerated until inoculation.
Method of Use: Allow medium to warm to room temperature prior to inoculation. Consult listed references for information concerning inoculation procedures. (4-6)
INTERPRETATION OF RESULTS
Production of gas within 48 +/- 2 hours of incubation is considered positive evidence of fermentation by coliform bacilli. Absence of gas production within the durham tube in 48 hours is considered a negative test for coliforms. Consult listed references for detailed results for the enumeration of coliforms using Brilliant Green Bile Broth. (3-5)
It may be necessary to invert the tube prior to inoculation if bubbles are trapped in the durham tube. Trapped bubbles that are not released may lead to false-positive results.
Turbidity alone is not indicative of a positive test for the presence of coliforms; turbidity with gas production is considered a positive test.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.
|Test Organisms||Inoculation Method*||Incubation||Results|
ATCC ® 25922
|A||18-48hr||35°C||Aerobic||Growth; gas bubble in durham tube|
ATCC ® 14028
|A||18-48hr||35°C||Aerobic||Growth; no gas bubble in durham tube|
ATCC ® 29212
User Quality Control
Brilliant Green Bile Broth should appear clear and light green in color.
Escherichia coli (ATCC ® 25922) growing in Brilliant Green Bile Broth (Cat. no. K09). The bubble in the Durham tube indicates gas production. Incubated aerobically for 24 hours at 35ºC.
Salmonella enterica (ATCC ® 14028) growing in Brilliant Green Bile Broth (Cat. no. K09). Incubated aerobically for 24 hours at 35ºC.
Enterococcus faecalis (ATCC ® 29212) inhibited in Brilliant Green Bile Broth (Cat. no. K09). Incubated aerobically for 24 hours at 35ºC.
1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.
2. Quality Assurance for Commercially Prepared Microbiological Culture Media , M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.
3. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.
4. American Public Health Association. Standard Methods for the Examination of Dairy Products, APHA, Washington, D.C.
5. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.
6. "Public Health Service Drinking Water Standards". 1962. Publication No. 956, U.S. Government Printing Office, Washington, D.C.
7. Association of Official Analytical Communities. Official Methods of Analysis . AOAC, Washington, D.C
ATCC is a registered trademark of the American Type Culture Collection.