BUTTERFIELD'S PHOSPHATE BUFFER

Cat. no. D125 Butterfield's Phosphate Buffer, Dilu-Lok II™ Vial, 25ml 50 vials/case
Cat. no. D590 Butterfield's Phosphate Buffer, Dilu-Lok II™ Vial, 90ml 50 vials/case
Cat. no. D599 Butterfield's Phosphate Buffer, Dilu-Lok II™ Vial, 99ml 50 vials/case
Cat. no. K109 Butterfield's Phosphate Buffer, 16x125mm Tube, 9ml 20 tubes/box
Cat. no. K119 Butterfield's Phosphate Buffer, 13ml Polypropylene
Screw Cap Tube, 9ml
20 tubes/box
Cat. no. K208 Butterfield's Phosphate Buffer, 16x125mm Tube, 9.9ml 20 tubes/box
Cat. no. K209 Butterfield's Phosphate Buffer, 20x125mm Tube, 9ml 20 tubes/box
Cat. no. U123 Butterfield's Phosphate Buffer, 500ml Wide Mouth Polycarbonate Bottle, 225ml 10 bottles/box
Cat. no. U124 Butterfield's Phosphate Buffer, 500ml Wide Mouth Polycarbonate Bottle, 190ml 10 bottles/box
Cat. no. U150 Butterfield's Phosphate Buffer, 500ml Polycarbonate Bottle, 450ml 10 bottles/box
Cat. no. U154 Butterfield's Phosphate Buffer, 500ml Polycarbonate Bottle, 400ml 10 bottles/box
Cat. no. U190 Butterfield's Phosphate Buffer, 500ml Polycarbonate Bottle, 500ml 10 bottles/box
Cat. no. U290 Butterfield's Phosphate Buffer, 125ml Polypropylene
Bottle, 100ml
12 bottles/box

INTENDED USE

Hardy Diagnostics Butterfield's Phosphate Buffer is used as a diluent for the preparation of dilutions in plate count and other laboratory processes.

This product is not intended to be used for the diagnosis of human disease.

SUMMARY

Butterfield's Phosphate Buffer is formulated according to the standard in the Compendium of Methods for the Microbiological Examination of Foods . (5) It is recommended as a general diluent in laboratory procedures by the Federal Drug Administration and in the Bacteriological Analytical Manual . (2) In addition, this product is described in Standard Methods for the Examination of Water and Wastewater for use in water testing. (1)

FORMULA

Ingredients per liter of deionized water:*

Monopotassium Phosphate 42.5mg

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-30ºC. away from direct light. Products should not be used if there are any signs of deterioration, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat and freezing.

PRECAUTIONS

PROCEDURE

Consult listed references for information on specimen collection and specific standard methods. (1-4)

General Dilution Guidelines:

1:10 Serial Dilutions
1. Using a sterile pipet, aliquot 10ml of test suspension to 90ml of the Dilu-Lok II™ diluent. Mix thoroughly. This yields a 1:10 dilution.

2. Use a second sterile pipet to aliquot 10ml of the 1:10 dilution prepared in step 1 into a second 90ml filled Dilu-Lok II™ diluent. Mix thoroughly. This yields a 1:100 dilution.

3. Continue aliquoting 10ml of subsequent dilutions into 90ml filled Dilu-Lok II™ diluents until the desired concentration of test sample is obtained. Each succeeding dilution increases by a factor of 10. A separate sterile pipet should be used with each dilution.

1:100 Serial Dilutions
1a. Using a sterile pipet, aliquot 1ml of test suspension to 99ml of the Dilu-Lok II™ diluent. Mix thoroughly. This yields a 1:100 dilution.

2a. Use a second sterile pipet to aliquot 1ml of the 1:100 dilution prepared in step 1a into a second 99ml filled Dilu-Lok II™ diluent. Mix thoroughly. This yields a 1:10,000 dilution.

3a. Continue aliquoting 1ml of subsequent dilutions into 99ml filled Dilu-Lok II™ diluents until the desired concentration of test sample is obtained. Each succeeding dilution increases by a factor of 100. A separate sterile pipet should be used with each dilution.

INTERPRETATION OF RESULTS

See listed references for interpretation of growth. (2,5)

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, sterile pipets, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Hardy Diagnostics tests Butterfield's Phosphate Buffer for sterility, toxicity, pH, and fill volume.

User Quality Control

PHYSICAL APPEARANCE

Butterfield's Phosphate Buffer should appear clear and colorless.

REFERENCES

1. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.

2. U.S. Food and Drug Administration. Bacteriological Analytical Manual. AOAC, Arlington, VA.
http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006949.htm.

3. Association of Official Analytical Chemists. Official Methods of Analysis sm , AOAC, Washington, D.C.

4. American Public Health Association. Standard Methods for the Examination of Dairy Products, APHA, Washington, D.C.

5. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.


040116sw