CRITERION™ AZIDE DEXTROSE BROTH
|Cat. no. C5050||CRITERION™ Azide Dextrose Broth||69.4gm|
|Cat. no. C5051||CRITERION™ Azide Dextrose Broth||500gm|
|Cat. no. C5052||CRITERION™ Azide Dextrose Broth||2kg|
|Cat. no. C5053||CRITERION™ Azide Dextrose Broth||10kg|
|Cat. no. C5054||CRITERION™ Azide Dextrose Broth||50kg|
Hardy Diagnostics CRITERION™ Azide Dextrose Broth is used to detect the presence of streptococci in water, shellfish, sewage, and milk.
This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.
Azide Dextrose Broth is recommended by the APHA for the isolation of fecal streptococci.(2) Various workers indicated that sodium azide could be used to inhibit gram-negative microorganisms while allowing the growth of gram-positive microorganisms, particularly Streptococcus spp. Azide Dextrose Broth has also been used for isolation of streptococci in foodstuffs and other specimens of sanitary significance as an indication of contamination.
Beef extract and tryptose serve as sources of carbon, nitrogen, vitamins and minerals. Dextrose serves as an energy source and sodium chloride is added to maintain the osmotic equilibrium. Sodium azide inhibits cytochrome oxidase in gram-negative bacteria.
|Gram weight per liter:||34.7gm/L|
|Animal Tissue Peptone||7.5gm|
Final pH 7.2 +/- 0.2 at 25ºC.
* Adjusted and/or supplemented as required to meet performance criteria.
STORAGE AND SHELF LIFE
Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.
Store the prepared media at 2-8ºC.
METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA
1. Suspend 34.7gm of the dehydrated culture media in 1 liter of distilled or deionized water.
2. Heat as needed to dissolve completely.
3. Autoclave at 121ºC. for 15 minutes.
PROCEDURE AND INTERPRETATION OF RESULTS
For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. K87.
CRITERION™ Azide Dextrose Broth is used to detect presumptive evidence of fecal contamination. Further biochemical testing must be done for confirmation.
For inoculum sizes of 10ml or larger, use double strength medium to prevent dilution of ingredients.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.
|Test Organisms||Inoculation Method*||Incubation||Results|
ATCC ® 29212
ATCC ® 25922
User Quality Control
CRITERION™ Azide Dextrose Broth powder should appear homogeneous, free-flowing, and light beige in color. Visually the single strength prepared broth should appear clear, and light to medium amber in color; double strength should appear clear, and medium to dark amber in color.
1. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.
2. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.
3. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.
4. American Public Health Association. Standard Methods for the Examination of Dairy Products, APHA, Washington, D.C.
ATCC is a registered trademark of the American Type Culture Collection.