CRITERION™ BURKHOLDERIA CEPACIA SELECTIVE AGAR (BCSA) BASE

Cat. no. C8880 CRITERION™ Burkholderia cepacia Selective Agar Base 121.2gm
Cat. no. C8881 CRITERION™ Burkholderia cepacia Selective Agar Base 500gm
Cat. no. C8882 CRITERION™ Burkholderia cepacia Selective Agar Base 2kg
Cat. no. C8883 CRITERION™ Burkholderia cepacia Selective Agar Base 10kg
Cat. no. C8884 CRITERION™ Burkholderia cepacia Selective Agar Base 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Burkholderia cepacia Selective Agar (BCSA) Base is used for the selective isolation of Burkholderia (Pseudomonas) cepacia. It is recommended for use as a general purpose growth media for the isolation and cultivation of Burkholderia (Pseudomonas) cepacia.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Burkholderia cepacia Selective Agar (BCSA) was developed by Henry, Campbell, LiPuma and Speert for the selective isolation of Burkholderia (Pseudomonas) cepacia.(2) Burkholderia cepacia is commonly isolated from cystic fibrosis patients. Correct identification of the organism is critical to patient care.(2) It was found that BCSA had a lower false-positivity rate than either Oxidation-Fermentation-Polymyxin-Bacitracin-Lactose (OFBPL) Agar or PC (Pseudomonas cepacia) Agar. This finding was confirmed later by Henry, Campbell, McGimpsey, Clarke, Louden, Burns, Roe, Vandamme and Speert.(1)

CRITERION™ BCSA Base contains peptones and sugars that supply nutrients for the growth of Burkholderia cepacia and other microorganisms. Crystal violet is added to inhibit growth of gram-positive organisms. Antimicrobics must be added to inhibit organisms other than Burkholderia cepacia.

FORMULA*

Gram weight per liter: 60.6gm/L
Sucrose 12.0gm
Lactose 12.0gm
Pancreatic Digest of Casein 13.0gm
Sodium Chloride 5.0gm
Crystal Violet 0.002gm
Yeast Extract 1.5gm
Phenol Red 0.08gm
Agar 17.0gm

Final pH 6.8 +/- 0.3 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light tan to beige.

Store the prepared culture media at 2-8ºC. away from light.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 60.6gm of the dehydrated culture media in one liter of distilled or deionized water. Stir to mix thoroughly.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool to 45-50ºC.

5. Aseptically add 10.0mg gentamicin, 600,000U polymyxin B, and 2.5mg vancomycin.

6. Stir to mix thoroughly.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. G09.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as antibiotics (gentamicin, polymyxin B, and vancomycin), autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Burkholderia ( Pseudomonas ) cepacia
ATCC ® 25416**
A 24-72hr 35°C Aerobic Growth; color change in media from red-orange to yellow
Pseudomonas aeruginosa
ATCC ® 27853**
B 24hr 35°C Aerobic Partial to complete inhibition
Staphylococcus aureus
ATCC ® 25923**
B 24hr 35°C Aerobic Partial to complete inhibition

** Recommended QC strains for User Quality Control according to the CLSI document M22 when applicable.

User Quality Control

Physical Appearance

Burkholderia cepacia Selective Agar (BCSA) Base powder should appear homogeneous, free-flowing, and light tan to beige in color. The prepared media should appear slightly opalescent, and reddish-orange in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

5. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

7. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

8. Henery, A.D., Campbell, E.M., LiPuma, J.J., Spreert, P.D. et al. 1996. Identification of Burkholderia cepacia Isolates from Patients with Cystic Fibrosis and Use of a Simple Selective Medium. J. Clin. Microbiol.; 35(8), 614-619. American Society for Microbiology.


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050916gr