CRITERION™ BRAIN HEART INFUSION (BHI) AGAR

Cat. no. C5120 CRITERION™ Brain Heart Infusion Agar 99.4gm
Cat. no. C5121 CRITERION™ Brain Heart Infusion Agar 500gm
Cat. no. C5122 CRITERION™ Brain Heart Infusion Agar 2kg
Cat. no. C5123 CRITERION™ Brain Heart Infusion Agar 10kg
Cat. no. C5124 CRITERION™ Brain Heart Infusion Agar 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Brain Heart Infusion Agar is a general purpose nutrient medium recommended for the cultivation and isolation of a variety of fastidious and nonfastidious microorganisms, including bacteria, yeasts, and mold.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Rosenow, by adding brain tissue to dextrose broth, discovered a medium useful in the cultivation of streptococci.(8) Formula modifications were made by various researchers who found the medium effective in the recovery of dental pathogens.(4,5,8) The medium was further modified by the addition of agar and a variety of supplements and enrichments which further enhanced the recovery of microorganisms.

The brain heart infusion, peptone and dextrose components of the medium provide the nutrients to BHI Agar. Organic nitrogen, carbon, sulfur, vitamins and trace substances are provided by the peptones and infusion. Dextrose provides the carbohydrate source for fermentative microorganisms. Disodium phosphate is added to the medium in order to maintain an optimal pH.

FORMULA

Gram weight per liter: 49.7gm/L
Pancreatic Digest of Casein 16.0gm
Brain Heart Infusion from Solids 8.0gm
Peptic Digest of Animal Tissue 5.0gm
Sodium Chloride 3.0gm
Disodium Phosphate 2.5gm
Dextrose 2.0gm
Agar 13.5gm

Final pH 7.4 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 49.7gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool to 45-50ºC. and aseptically add enrichments, if desired.

5. Dispense into sterile containers.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. W15.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Escherichia coli
ATCC® 25922
A 24-48hr 35°C Aerobic Growth
Staphylococcus aureus
ATCC® 25923
A 24-48hr 35°C Aerobic Growth
Staphylococcus epidermidis
ATCC® 12228
A 18-24hr 35°C Aerobic Growth

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Brain Heart Infusion Agar powder should appear homogeneous, free-flowing, and beige in color. The prepared media should appear clear, and light to medium amber in color.

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Falk, C.R., et al. 1939. J. Bacteriol.; 37:121.

4. Hayden, R.L. 1932. Arch. Internal Med.; 32:828.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

6. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

7. Hitchens, A.P. 1921. J. Infect. Disease; 29:230.

8. Rosenow, E.C. 1919. J. Dental Research; 1:205.

9. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

ATCC is a registered trademark of the American Type Culture Collection.

071516vr