CRITERION™ BRAIN HEART INFUSION (BHI) BROTH

Cat. no. C5140 CRITERION™ Brain Heart Infusion Broth 74gm
Cat. no. C5141 CRITERION™ Brain Heart Infusion Broth 500gm
Cat. no. C5142 CRITERION™ Brain Heart Infusion Broth 2kg
Cat. no. C5143 CRITERION™ Brain Heart Infusion Broth 10kg
Cat. no. C5144 CRITERION™ Brain Heart Infusion Broth 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Brain Heart Infusion Broth is a general purpose nutrient medium recommended for the cultivation and isolation of a variety of fastidious and nonfastidious microorganisms, including bacteria, yeasts, and mold.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Brain Heart Infusion Broth was first described by Rosenow.(8) Pieces of brain tissue were added to dextrose broth and used for the cultivation of streptococci. Hayden revised Rosenow's procedure by adding crushed marble to the medium and reported favorable growth of organisms from dental pathogens.(3,4,7) Brain Heart Infusion Agar is a modification of the media described by Rosenow and Hayden in which calf brains have replaced the brain tissue and disodium phosphate has replaced the calcium carbonate buffer. Furthermore, it has been shown that the formulation which employs calf brains, equivalent in nutritional value, provides a clearer medium.

FORMULA

Gram weight per liter: 37.0gm/L
Pancreatic Digest of Casein 14.5gm
Brain Heart Infusion from Solids 10.0gm
Peptic Digest of Animal Tissue 5.0gm
Sodium Chloride 5.0gm
Disodium Phosphate 2.5gm
Dextrose 2.0gm

Final pH 7.4 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 37.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat as necessary to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool to 45-50ºC. and aseptically add enrichments, if desired.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. K27.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Escherichia coli
ATCC ® 25922
A 24-48hr 35°C Aerobic Growth; broth becomes turbid
Staphylococcus aureus
ATCC ® 25923
A 24-48hr 35°C Aerobic Growth; broth becomes turbid

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Brain Heart Infusion Broth powder should appear homogeneous, free-flowing, and beige in color. The prepared media should appear clear, and light to medium amber in color.

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Falk, C.R., et al. 1939. J. Bacteriol.; 37:121.

4. Hayden, R.L. 1932. Arch. Internal Med.; 32:828.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

6. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

7. Hitchens, A.P. 1921. J. Infect. Disease; 29:230.

8. Rosenow, E.C. 1919. J. Dental Research; 1:205.

9. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.


ATCC is a registered trademark of the American Type Culture Collection.

050916gr