Cat. no. C5100 CRITERION™ Beef Extract Powder 100gm
Cat. no. C5101 CRITERION™ Beef Extract Powder 500gm
Cat. no. C5102 CRITERION™ Beef Extract Powder 2kg
Cat. no. C5103 CRITERION™ Beef Extract Powder 10kg
Cat. no. C5104 CRITERION™ Beef Extract Powder 50kg


Hardy Diagnostics CRITERION™ Beef Extract Powder is prepared and standardized for use in microbiological culture media, where it is generally used to replace infusions of meat. It is standard in its composition and reaction and does not require adjustment of reaction or filtration. For many years, beef extract media have been recommended as standard for use in routine bacteriological examination of water, milk, and other materials where it is important to have media of uniform composition.

This is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.


CRITERION™ Beef Extract Powder is recommended for use as a nutrient at 0.3-0.5% concentration in various culture media. Beef Extract can be used in fermentation studies since it is free of fermentable substrates. A 0.3% solution after autoclaving is clear and has a pH of 6.7-6.9.


Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original dark tan.



Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

Physical Appearance

CRITERION™ Beef Extract Powder should appear homogeneous, free-flowing, and dark tan in color. A 0.3% solution of Beef Extract Powder should appear clear, and light to medium amber in color, with no precipitate or debris.


1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

4. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

5. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

6. Marshall, R.T., ed. 1992. Standard Methods for the Examination of Dairy Products, 16th ed. APHA, Washington, D.C.

7. Greenberg, A.E., et al. (ed.). 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. APHA, Washington, D.C.