CRITERION™ BiGGY AGAR
|Cat. no. C5160||CRITERION™ BiGGY Agar||98gm|
|Cat. no. C5161||CRITERION™ BiGGY Agar||500gm|
|Cat. no. C5162||CRITERION™ BiGGY Agar||2kg|
|Cat. no. C5163||CRITERION™ BiGGY Agar||10kg|
|Cat. no. C5164||CRITERION™ BiGGY Agar||50kg|
Hardy Diagnostics CRITERION™ BiGGY Agar (Bismuth Sulfite Glucose Glycine Yeast Agar) is a selective and differential medium used in the isolation and presumptive identification of Candida spp.
This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.
BiGGY Agar was developed by Nickerson in 1953 following a study of sulfite reduction by Candida species. Nickerson found many yeast capable of reducing bismuth sulfite to bismuth sulfide when grown on an acidic or neutral medium containing the reducing substrate. Substrate reduction was noted by the production of brown to black pigmented colonies, a result of sulfide combining with bismuth.(1-7)
Bismuth sulfite inhibits bacterial growth, thereby enabling the recovery of isolated colonies of Candida. Candida spp. reduce the bismuth sulfite resulting in pasty brown to black colonies. Some Candida species present as brown to black colonies surrounded by zones of dark precipitate in the medium. Dextrose and yeast extract provide the nutrients in the formulation.
|Gram weight per liter:||49.0gm/L|
|Bismuth Ammonium Citrate||5.0gm|
Final pH 6.8 +/- 0.2 at 25ºC.
* Adjusted and/or supplemented as required to meet performance criteria.
STORAGE AND SHELF LIFE
Store the sealed bottle(s) containing dehydrated culture medium at 2-30 degrees C. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.
Store the prepared media in plates at 2-8ºC.
PROCEDURE AND INTERPRETATION OF RESULTS
For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. G17.
Bacteria and yeast like fungi are mostly inhibited on this media, however, if break-through occurs they can be easily differentiated by microscopic examination.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.
|Test Organisms||Inoculation Method*||Incubation||Results|
ATCC ® 60193
|A||24-72hr||15-30°C||Aerobic||Growth; smooth tan or brown to rust colonies, no diffusion of color|
ATCC ® 14243
|A||24-72hr||15-30°C||Aerobic||Growth; flat wrinkled reddish-brown colonies, yellow diffusion of color in medium, silver sheen|
ATCC ® 750
|A||24-72hr||15-30°C||Aerobic||Growth; smooth brown to black colonies|
ATCC ® 25922
|B||24hr||15-30°C||Aerobic||Partial to complete inhibition|
User Quality Control
CRITERION™ BiGGY Agar powder should appear homogeneous, free-flowing, and light beige in color. The prepared media should appear opalescent with moderate precipitate evenly suspended throughout the medium, and slightly white in color.
1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.
2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.
3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.
4. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.
5. Larone, D.H. Medically Important Fungi: A Guide to Identification, American Society for Microbiology. Washington, D.C.
6. Nickerson. 1953. Journal of Infectious Diseases; 93:43.
7. Atlas, Ronald M. 1993. Handbook of Microbiological Media, CRC Press, Inc.
ATCC is a registered trademark of the American Type Culture Collection.