CRITERION™ BRILLIANT GREEN AGAR

Cat. no. C5240 CRITERION™ Brilliant Green Agar 116gm
Cat. no. C5241 CRITERION™ Brilliant Green Agar 500gm
Cat. no. C5242 CRITERION™ Brilliant Green Agar 2kg
Cat. no. C5243 CRITERION™ Brilliant Green Agar 10kg
Cat. no. C5244 CRITERION™ Brilliant Green Agar 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Brilliant Green Agar is recommended for the selective enrichment of Salmonella spp. other than S. typhi and S. paratyphi.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Kristensen et al., in 1925, first described use of Brilliant Green Agar as a primary plating medium for the isolation of Salmonella spp.(8) Kauffman modified the formula in 1935.(9)

The current formulation incorporates phenol red as the pH indicator and brilliant green as an inhibitory agent that acts against gram-positive organisms and gram-negative bacilli. Organisms that ferment lactose and/or sucrose exhibit yellow to yellow-green colonies surrounded by a yellow-green zone. Salmonella appears as red to pink-white colonies surrounded by a red zone in the medium.

CRITERION™ Brilliant Green Agar is not recommended for the selective isolation of Salmonella typhi.

FORMULA

Gram weight per liter: 58.0gm/L
Lactose 10.0gm
Sucrose 10.0gm
Sodium Chloride 5.0gm
Pancreatic Digest of Casein 5.0gm
Peptic Digest of Animal Tissue 5.0gm
Yeast Extract 3.0gm
Phenol Red 0.08gm
Brilliant Green 12.5mg
Agar 20.0gm

Final pH 6.9 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared tubed media at 2-30ºC. Plated media should be stored at 2-8 degrees C.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 58.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult list of references or refer to the prepared media Instructions for Use (IFU) for Cat. No. G75.

LIMITATIONS

The recovery of many Salmonella spp. is greatly jeopardized if stool specimens remain unpreserved for more than three hours before processing. If there is to be a delay in processing, the specimen should be inoculated onto an appropriate transport medium in order to maintain viability of the organisms.

Color variations from red to pink-white may occur in colonies of Salmonella spp. Variations in color are dependent upon the length of incubation and the strain of the organism.

Other non-lactose-fermenting or slow lactose-fermenting organisms may grow on the agar and imitate the enteric pathogens.

Brilliant Green Agar is not recommended for isolation of Salmonella typhi, Salmonella paratyphi, and Shigella spp.

The medium will become discolored if exposed to light.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Salmonella enterica
ATCC ® 14028
A 18-24hr 35°C Aerobic Growth; red to pink-white colonies with red zones
Escherichia coli
ATCC ® 25922
B 18-24hr 35°C Aerobic Partial to complete inhibition; small yellow to yellow-green colonies

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Brilliant Green Agar powder should appear homogeneous, free-flowing, and light beige in color. The prepared media should appear slightly opalescent, and brownish-orange in color.

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

4. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

5. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

6. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

7. Sack, R.B., et al. 1980. Cumitech 12; American Society for Microbiology, Washington, D.C.

8. Kristensen, M., et al. 1925. Br. J. Exp. Pathol.; 6:291.

9. Kauffman, F. 1935. Z. Hyg. Infektionskr.; 117:26.


ATCC is a registered trademark of the American Type Culture Collection.

051616gr