CRITERION™ BRILLIANT GREEN BILE BROTH

Cat. no. C5250 CRITERION™ Brilliant Green Bile Broth 80gm
Cat. no. C5251 CRITERION™ Brilliant Green Bile Broth 500gm
Cat. no. C5252 CRITERION™ Brilliant Green Bile Broth 2kg
Cat. no. C5253 CRITERION™ Brilliant Green Bile Broth 10kg
Cat. no. C5254 CRITERION™ Brilliant Green Bile Broth 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Brilliant Green Bile Broth is recommended for the detection of coliforms in water and other samples.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

The formulation for Brilliant Green Bile Broth was developed by the Association of Official Analytical Chemistry and the American Public Health Association.(3,5) The basal medium is composed of peptone, which contains 2% bile, and brilliant green dye. The bile is inhibitory to gram-positive flora while the brilliant green dye inhibits selected gram-negative bacilli. Lactose-fermenting organisms that are resistant to the inhibitors are detected by the production of gas. Gas production is noted by the appearance of bubbles in the durham tube.

FORMULA

Gram weight per liter: 40.0gm/L
Oxbile (Oxgall) 20.0gm
Pancreatic Digest of Gelatin 10.0gm
Lactose 10.0gm
Brilliant Green 13.3mg

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original greenish-beige.

Store the prepared culture media at 2-30ºC. Tubed media with slips caps are stored at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 40.0gm of the dehydrated culture media in 1 liter of distilled or deionized water. Stir to mix thoroughly.

2. Heat as necessary to dissolve completely. Avoid overheating.

3. Dispense into tubes containing durham tubes.

4. Sterilize in the autoclave at 121ºC. for 12-15 minutes. Heate sensitive product. Depending on autoclave used, time or temperature adjustment may be necessary.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed referencs or refer to the prepared media Instructions for Use (IFU) for Cat. No. K09.

LIMITATIONS

It may be necessary to invert the tube prior to inoculation if bubbles are trapped in the durham tube. Trapped bubbles that are not released may lead to false-positive results.

Turbidity alone is not indicative of a positive test for the presence of coliforms; turbidity with gas production is considered a positive test.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Escherichia coli
ATCC ® 25922
A 18-48hr 35°C Aerobic Growth; gas bubble in durham tube
Salmonella enterica
ATCC ® 14028
A 18-48hr 35°C Aerobic Growth; no gas bubble in durham tube
Enterococcus faecalis
ATCC ® 29212
B 18-48hr 35°C Aerobic Inhibited

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Brilliant Green Bile Broth powder should appear homogeneous, free-flowing, and greenish-beige in color. The prepared media should appear clear, and light green in color.

REFERENCES

1. Compendium of Methods for the Microbiological Examination of Food. American Public Health Association, Washington, D.C.

2. American Public Health Association. Standard Methods for the Examination of Dairy Products, APHA, Washington, D.C.

3. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.

4. "Public Health Service Drinking Water Standards," Publication No. 956. 1962. U.S. Government Printing Office, Washington, D.C.

5. Williams, ed. 1984. Official Methods of Analysis of the Association of Official Analytical Chemists, 14th ed., AOAC, Arlington, VA.


ATCC is a registered trademark of the American Type Culture Collection.

051616gr