CRITERION™ Brilliant Green Agar, Modified

Cat. no. C8690 CRITERION™ Brilliant Green Agar, Modified 113.2gm
Cat. no. C8691 CRITERION™ Brilliant Green Agar, Modified 500gm
Cat. no. C8692 CRITERION™ Brilliant Green Agar, Modified 2kg
Cat. no. C8693 CRITERION™ Brilliant Green Agar, Modified 10kg
Cat. no. C8694 CRITERION™ Brilliant Green Agar, Modified 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Brilliant Green Agar, Modified is recommended for the selective isolation of Salmonella spp., other than S. typhi, from a variety of specimen types.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Brilliant Green Agar, Modified was formulated by Edel and Kamplemacher of the Netherlands Institute for Public Health, Utrecht and originally proposed as a selective medium for the isolation of Salmonella from pig feces and minced meat.(8,9) The medium has been widely assessed in Europe and used in the Standard European Community and by the International Standards Organization.(7,10) More selective than Deoxycholate Citrate Agar and other brilliant green media, Brilliant Green Agar, Modified inhibits the growth of Pseudomonas aeruginosa and Proteus spp., which may resemble the growth of Salmonella spp., thus reducing the burden of colony identification.

Brilliant Green Agar, Modified is recommended for the selective isolation of Salmonella spp., other than S.typhi, from clinical and non-clinical specimens. The medium is appropriate for subculture from selective enrichment media.

FORMULA*

Gram weight per liter: 56.6gm/L
Lactose 10.0gm
Sucrose 10.0gm
Casein Peptone 5.0gm
Animal Tissue Peptone 5.0gm
Gelatin Peptone 5.0gm
Sodium Chloride 5.0gm
Yeast Extract 3.0gm
Disodium Phosphate 1.0gm
Phenol Red 0.09gm
Monopotassium Phosphate 0.52gm
Brilliant Green 4.7mg
Agar 12.0gm

Final pH 6.9 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30 degrees C. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 56.6gm of the dehydrated culture media in one liter of distilled or deionized water. Stir to mix thoroughly.

2. Heat to boiling to dissolve completely. Do not overheat.

3. Cool to 45-50ºC. and dispense desired volume into sterile containers.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, other culture media such as Buffered Peptone Water (Cat. no. U142), Tetrathionate Broth Base (Cat. no. K65 or U165), as well as supplements, such as Iodine-Iodide Solution (Cat. no. Z129 or Z139), etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Salmonella typhimurium
ATCC ® 14028
A 18-24hr 35°C Aerobic Growth; red to pink-white colonies with red zones
Escherichia coli
ATCC ® 25922
B 18-24hr 35°C Aerobic Partial to complete inhibition; small yellow to yellow-green colonies

User Quality Control

Physical Appearance

CRITERION™ Brilliant Green Agar, Modified powder should appear homogeneous, free-flowing, and light beige in color. The prepared medium should appear slightly opalescent, and brownish-orange in color.

REFERENCES

1. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.

2. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.

3. Anon. 1975. Meat and meat products - detection of salmonellae (reference method). International Organization for Standardization. Geneva, Switzerland.

4. Anon. 1981. Microbiology - General Guidance on Methods for the Detection of Salmonella Ref. method ISO 6579-1981(E). International Organization for Standardization. Geneva, Switzerland.

5. Anon. 2001. Milk and Milk Productss - Detection of Salmonella spp. ISO 6785. International Organization for Standardization. Geneva, Switzerland./pr>

6. Atlas, R.M. 2010. Handbook of Microbiological Media, 4th ed. CRC Press, Inc. Boca Raton, FL.

7. Corry, J.E.L, G.D.W. Curtis, and R.M. Baird. 2003. Handbook of Culture Media for Microbiology. Elsevier Science B.V. Amsterdam, The Netherlands.

8. Edel, W. and E.H. Kamplemacher. 1968. Comparative studies on Salmonella isolation in eight European laboratories. Bull. Wld. Hlth. Org.;39:487-491.

9. Edel, W. and E.H. Kamplemacher. 1969. Salmonella infections in nine European lanoratories using a standard technique. Bull. Wld. Hlth. Org.; 41:297-306.

10. Parry, P.T., L. Haysom, N.L. Thomas, and R. Davis.1982. A Manual of Recommended Methods for the Microbiological Examination of Poultry and Poultry Products. British Poultry Meat Association. London, U.K.

11. Read, JR., R.B. and A.L. Reyes. 1968. Variation in plating efficiency of salmonellae on eight lots of Brilliant Green Agar. Appl. Microbiol; 16(5):746-748.

12. Reed, G.H. 1993. Foodborne illness (Part 2): Salmonellosis. Dairy, Food, Environ. San 13:706.

13. Vassilliadis, P, J. Trichopoulos, V.K. Papadakis and Ch. Serie. 1979. Brilliant Green Deoxycholate Agar as an improved selective medium for the isolation of Salmonella. Ann. Soc. belge. Med. trop.; 59:117-120.

ATCC is a registered trademark of the American Type Culture Collection.


051616gr