CRITERION™ BRUCELLA AGAR

Cat. no. C5300 CRITERION™ Brucella Agar 86gm
Cat. no. C5301 CRITERION™ Brucella Agar 500gm
Cat. no. C5302 CRITERION™ Brucella Agar 2kg
Cat. no. C5303 CRITERION™ Brucella Agar 10kg
Cat. no. C5304 CRITERION™ Brucella Agar 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Brucella Agar is used for the cultivation of Brucella species, and other fastidious organisms.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Brucella Agar is prepared according to APHA formula for Albimi Broth, which is used for the isolation of Brucella species.(2) Brucellosis is a zoonotic disease with a domestic-animal reservoir. Transmission by milk, milk products, meat and direct contact with infected animals is the usual route of exposure.(1)

CRITERION™ Brucella Agar contains pancreatic digest of casein, peptic digest of animal tissue, and yeast extract as sources of nutrients. Dextrose is added as a carbon source in the media. Various growth factors and additives can be added aseptically to the media after it has been autoclaved. Adding hemin, vitamin K, sheep or horse blood, provide nutrients for more fastidious organisms. Antibiotics may be added to make the media selective.

Brucella Agar is also recommended for use in agar dilution susceptibility tests for anaerobes.(1) Additionally, Standard Methods for the Examination of Dairy Products recommends Brucella Agar for the cultivation of Brucella species from dairy samples.(2)

FORMULA

Gram weight per liter: 43.0gm/L
Pancreatic Digest of Casein 10.0gm
Peptic Digest of Animal Tissue 10.0gm
Yeast Extract 2.0gm
Sodium Chloride 5.0gm
Dextrose 1.0gm
Sodium Bisulfite 0.1gm
Agar 15.0gm

Final pH 7.0 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 43.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes. Do not overheat.

4. Cool to 45-50ºC. and aseptically add enrichments, if desired.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. A30 which contains sheep blood, hemin, and vitamin K.

LIMITATIONS

Brucella Agar is a general purpose growth media. Colonies growing on Brucella Agar will require further biochemical and/or serological testing for complete identification.

Since the nutritional requirements of organisms vary, some strains may be encountered that fail to grow or grow poorly on this medium.

Hemolytic reactions of organisms may vary on this medium depending on the type of animal blood used. For example, some group D streptococci exhibit beta-hemolysis on media containing horse blood but not on sheep blood and may be mistaken for group A streptococci.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Bacteroides fragilis
ATCC ® 25285
A 24-72hr 35°C Anaerobic Growth
Clostridium perfringens
ATCC ® 13124
A 24-72hr 35°C Anaerobic Growth

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Brucella Agar powder should appear homogeneous, free-flowing, and light beige in color. The prepared media should appear slightly hazy, and light amber in color.

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Marshall, R.T., ed. 1992. Standard Methods for the Examination of Dairy Products, 16th ed. APHA, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

5. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

6. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

7. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.


ATCC is a registered trademark of the American Type Culture Collection.

050916gr