CRITERION™ BRUCELLA BROTH

Cat. no. C5310 CRITERION™ Brucella Broth 56gm
Cat. no. C5311 CRITERION™ Brucella Broth 500gm
Cat. no. C5312 CRITERION™ Brucella Broth 2kg
Cat. no. C5313 CRITERION™ Brucella Broth 10kg
Cat. no. C5314 CRITERION™ Brucella Broth 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Brucella Broth is used for the cultivation of Brucella species, and other fastidious organisms.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

CRITERION™ Brucella Broth, used for cultivation of Brucella and other fastidious organisms, is based upon the APHA formulation for Albimi Broth.(3) Brucella Broth is recommended for the isolation of Brucella species from blood cultures.(1,4) Additionally, Brucella Broth is specified in the Compendium of Methods for the Microbiological Examination of Food.(10)

CRITERION™ Brucella Broth contains pancreatic digest of casein, peptic digest of animal tissue, and yeast extract as sources of nutrients. Dextrose is added as a carbon source in the media. Various growth factors, such as hemin and vitamin K, can be added aseptically to the medium after it has been autoclaved, to provide additional nutrients for more fastidious organism. Antibiotics can also be added to make the medium more selective.

FORMULA

Gram weight per liter: 28.0gm/L
Pancreatic Digest of Casein 10.0gm
Peptic Digest of Animal Tissue 10.0gm
Yeast Extract 2.0gm
Sodium Chloride 5.0gm
Dextrose 1.0gm
Sodium Bisulfite 0.1gm

Final pH 7.0 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 28.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat as necessary to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool to 45-50ºC. and aseptically add enrichments or antibiotics, if desired.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. K218.

LIMITATIONS

Brucella Broth is a general purpose growth media. Organisms growing in Brucella Broth will require further biochemical and/or serological testing for complete identification.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Bacteroides fragilis
ATCC ® 25285
A 24-72hr 35°C Anaerobic Growth
Clostridium perfringens
ATCC ® 13124
A 24-72hr 35°C Anaerobic Growth
Streptococcus pyogenes
ATCC ® 19615
A 24-72hr 35°C CO 2 ** Growth

** Atmosphere of incubation is enriched with 5-10% CO2.

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Brucella Broth powder should appear homogeneous, free-flowing, and light beige in color. The prepared media should appear clear to slightly hazy, and light amber in color.

REFERENCES

1. Baron, E.J., L.R. Peterson and S.M. Finegold. 1994. Bailey and Scott's Diagnostic Microbiology, 9th ed. Mosby-Year Book, Inc., St. Louis, MO.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Hausler, W.J. 1976. Standard Methods for the Examination of Dairy Products, 14th ed. American Public Health Association, Washington, D.C.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

5. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

6. Marshall, R.T., ed. 1992. Standard Methods for the Examination of Dairy Products, 16th ed. APHA, Washington, D.C.

7. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

8. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

9. Vanderzant, C. and D.F. Splittstoesser. 1992. Compendium of Methods for the Microbiological Examination of Food, 3rd ed. APHA, Washington, D.C.


ATCC is a registered trademark of the American Type Culture Collection.

051616gr