CRITERION™ BUFFERED PEPTONE WATER

Cat. no. C5320 CRITERION™ Buffered Peptone Water 40gm
Cat. no. C5321 CRITERION™ Buffered Peptone Water 500gm
Cat. no. C5322 CRITERION™ Buffered Peptone Water 2kg
Cat. no. C5323 CRITERION™ Buffered Peptone Water 10kg
Cat. no. C5324 CRITERION™ Buffered Peptone Water 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Buffered Peptone Water is intended to aid in the recovery of injured Salmonella species from foods and other samples prior to selective enrichment and isolation.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Salmonella that may be present in a food product can be sublethally injured by food processing techniques. These injured Salmonella may not be recovered by direct selection techniques. Buffered Peptone Water is a non-selective preenrichment broth that promotes the recovery of those bacteria that may be injured.

Peptone in the media supplies nitrogenous compounds needed for the growth of bacteria. The phosphate salts provide buffering capacity to maintain the pH. Maintenance of the pH is important when attempting to recover injured bacteria, a low pH can be detrimental to the repair of the damaged cells.

FORMULA

Gram weight per liter: 20.0gm/L
Peptone 10.0gm
Sodium Chloride 5.0gm
Disodium Phosphate 3.5gm
Monopotassium Phosphate 1.5gm

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared culture medium at 2-30ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 20.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. K107.

LIMITATIONS

Competing flora in the test sample can affect the recovery and may overgrow Salmonellae.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Salmonella enterica
ATCC ® 14028
A 18-24hr 35°C Aerobic Growth when subcultured to XLD
Escherichia coli
ATCC ® 25922
B 18-24hr 35°C Aerobic Partial to complete inhibition when subcultured to XLD

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Buffered Peptone Water powder should appear homogeneous, free-flowing, and light beige in color. The prepared media should appear clear, and colorless.

REFERENCES

1. FDA. 1995. Bacteriological Analytical Manual, 8th ed. FDA.

2. Marshall, R.T., ed. 1992. Standard Methods for the Examination of Dairy Products, 16th ed. APHA, Washington, D.C.

3. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed.. APHA, Washington, D.C.

4. Sadovski, A.Y. 1977. J. Food Technology; 12:85-91.

5. Juven, B.J, N. Cox, J.S. Bailey, J.E. Thomson, O.W. Charles and J.V. Schutze. 1984. Recovery of Salmonella from artificially contaminated poultry feeds in non-selective and selective broth media. Jour. of Food Prot.; 47:299-302.


ATCC is a registered trademark of the American Type Culture Collection.

051616gr