CRITERION™ CASEIN, ACID HYDROLYSATE

Cat. no. C5340 CRITERION™ Casein, Acid Hydrolysate 100gm
Cat. no. C5341 CRITERION™ Casein, Acid Hydrolysate 500gm
Cat. no. C5342 CRITERION™ Casein, Acid Hydrolysate 2kg
Cat. no. C5343 CRITERION™ Casein, Acid Hydrolysate 10kg
Cat. no. C5344 CRITERION™ Casein, Acid Hydrolysate 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Casein, Acid Hydrolysate is primarily for use in preparing Mueller Hinton Agar, Mueller Hinton Broth, and other culture media.

This is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Casein, Acid Hydrolysate is an hydrochloric acid hydrolysate of casein. Hydrolysis with acid is non-specific, attacking all peptide bonds and degrading proteins and polypeptides to low chain length peptides and amino acids. In the analytical data this is shown by a high amino nitrogen (compared to enzymatic digests) and the presence of high levels of sodium chloride. The latter is caused by neutralization of hydrochloric acid with sodium hydroxide.

Acid hydrolysis destroys tryptophan and partially destroys cystine, serine and threonine. Asparagine and glutamine are converted to their acidic forms and vitamins are destroyed.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

PRECAUTIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

PHYSICAL APPEARANCE

CRITERION™ Casein, Acid Hydrolysate powder should appear homogeneous, free-flowing, and light beige in color. The pH of a 2% solution of CRITERION™ Casein, Acid Hydrolysate is 7.0.

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

4. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

5. Atlas, R.M. 1997. Handbook of Microbiological Media, 2nd ed. CRC Press, Boca Raton, FL.

6. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.


051616gr