CRITERION™ DEXTROSE BROTH

Cat. no. C5600 CRITERION™ Dextrose Broth 46gm
Cat. no. C5601 CRITERION™ Dextrose Broth 500gm
Cat. no. C5602 CRITERION™ Dextrose Broth 2kg
Cat. no. C5603 CRITERION™ Dextrose Broth 10kg
Cat. no. C5604 CRITERION™ Dextrose Broth 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Dextrose Broth is recommended for the cultivation of fastidious microorganisms and for the detection of gas from enteric bacilli.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

CRITERION™ Dextrose Broth is used for the isolation of fastidious organisms and specimens containing a low inoculum due to its highly nutritious formula. Dextrose Broth, with the addition of 0.1-0.2% agar, can facilitate anaerobic growth and aids in dispersion of reducing substances and CO2 formed in the environment(1) This low concentration of agar allows for aerobic growth in the clear upper zone and for microaerophilic and anaerobic growth in the lower agar zones. Dextrose Broth is specified in the Compendium of Methods for the Microbiological Examination of Foods(2)

Meat peptone, beef extract and casein peptone serve as sources of carbon, nitrogen, vitamins and minerals. Dextrose serves as an energy source and sodium chloride is added to maintain the osmotic equilibrium.

FORMULA

Gram weight per liter: 23.0gm/L
Pancreatic Digest of Casein 5.0gm
Peptic Digest of Animal Tissue 5.0gm
Dextrose 5.0gm
Sodium Chloride 5.0gm
Beef Extract 3.0gm

Final pH 7.2 +/- 0.2 at 25 degrees C.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light tan.

Store the prepared media at 2-30ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 23.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Sterilize in the autoclave at 121ºC. for 15 minutes.

3. Cool to 45-50ºC.

4. Dispense into tubes containing durham tubes.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Escherichia coli
ATCC ® 25922
A 18-48hr 35°C Aerobic Growth; gas produced
Staphylococcus aureus
ATCC ® 25923
A 18-48hr 35°C Aerobic Growth; no gas produced
Streptococcus pyogenes
ATCC ® 19615
A 18-48hr 35°C Aerobic Growth; no gas produced

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Dextrose Broth powder should appear homogeneous, free-flowing, and light tan in color. The prepared media should appear clear, and light to medium amber in color.

REFERENCES

MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

2. Vanderzant, C. and D.F. Splittstoesser. 1992. Compendium of Methods for the Microbiological Examination of Food, 3rd ed. American Public Health Association, Washington, D.C.


ATCC is a registered trademark of the American Type Culture Collection.

052316gr