CRITERION™ EE Broth, Mossel

Cat. no. C8710 CRITERION™ EE Broth, Mossel 90gm
Cat. no. C8711 CRITERION™ EE Broth, Mossel 500gm
Cat. no. C8712 CRITERION™ EE Broth, Mossel 2kg
Cat. no. C8713 CRITERION™ EE Broth, Mossel 10kg
Cat. no. C8714 CRITERION™ EE Broth, Mossel 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ EE Broth, Mossel is recommended for the selective enrichment and isolation of Enterobacteriaceae from foods. This medium meets the harmonized United States Pharmacopeia (USP), European Pharmacopoeia (EP) and Japanese Pharmacopoeia (JP) standards for use as an enrichment medium for the microbial examination of nonsterile products.(5,6)

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

The enumeration of Enterobacteriaceae is of great concern in monitoring the sanitary quality of foods. Most Enterobacteriaceae are easily injured during food-processing procedures, especially by exposure to low temperatures, sub-marginal heat, drying, radiation, preservatives or sanitizers.(2) The ability to successfully recover these organisms depends upon the proper resuscitation of damaged or sub-lethally injured cells.

CRITERION™ EE Broth, Mossel is made according to the formula developed by Mossel, Visser and Cornelissen.(3) The formula contains dextrose to facilitate the growth of most Enterobacteriaceae, thus promoting the detection of Salmonella and other non-lactose fermenting bacteria. CRITERION™ EE Broth, Mossel should be used as an enrichment broth, followed by plating to a selective medium such as Violet Red Bile Agar or Violet Red Bile Agar with Glucose. In addition, the medium conforms to the harmonized USP/EP/JP requirements for the detection of bile-tolerant, gram-negative microorganisms.(5,6)

CRITERION™ EE Broth, Mossel contains peptones as a source of nitrogen, vitamins and amino acids. Dextrose provides a carbon source. Disodium phosphate and monopotassium phosphate are included as buffering agents, while brilliant green and oxbile (oxgall) are included as selective agents.

FORMULA*

Gram weight per liter: 45.0gm/L
Oxbile (Oxgall) 20.0gm
Gelatin Peptone 10.0gm
Disodium Phosphate 8.0gm
Dextrose 5.0gm
Monopotassium Phosphate 2.0gm
Brilliant Green 0.015gm

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light green.

Store the prepared culture media at 2-30 degrees C.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 45.0gm of the dehydrated culture media in one liter of distilled or deionized water. Stir to mix thoroughly.

2. Heat as necessary to dissolve completely.

3. Heat at 100ºC. for 30 minutes. Cool immediately.

4. DO NOT AUTOCLAVE.

5. Aseptically dispense desired volume into sterile containers.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. K191 or U391.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, other culture media such as Violet Red Bile Agar (Cat. no. G78) or Violet Red Bile Agar with Glucose (Cat. no. G178), and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Pseudomonas aeruginosa
ATCC ® 9027
J 24-48hr 35°C Aerobic Growth; no color change
Escherichia coli
ATCC ® 8739
J 24-48hr 35°C Aerobic Growth; color change to yellow
Staphylococcus aureus
ATCC ® 6538
B 48hr 35°C Aerobic Inhibited; no color change

User Quality Control

Physical Appearance

CRITERION™ EE Broth, Mossel powder should appear homogeneous, free-flowing, and light green in color. The prepared medium should appear clear, and emerald green in color.

REFERENCES

1. Downes and Ito (ed.). 2001. Compendium of Methods for the Microbiological Examination of Foods, 4th ed. American Public Health Association, Washington, D.C.

2. Hartman and Minnich. 1981. Automation for Rapid Detection of Salmonellae in Foods. J. Food Prot. 44:385.

3. Mossel, D.A.A., M. Visser and A.M.R. Cornelissen. 1963. The Examination of Foods for Enterobacteriaceae using a Test of the Type Generally Adopted for the Detection of Salmonellae. J. Appl. Bacteriol.; 26:444.

4. U.S. Food and Drug Administration. Bacteriological Analytical Manual. AOAC, Arlington, VA.
http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006949.htm.

5. The Official Compendia of Standards. USP General Chapter <61> Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests. USP-NF. United States Pharmacopeial Convention Inc., Rockville, MD.

6. The Official Compendia of Standards. USP General Chapter <62> Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms. USP-NF. United States Pharmacopeial Convention Inc., Rockville, MD.


ATCC is a registered trademark of the American Type Culture Collection.

052316gr