CRITERION™ GC AGAR BASE

Cat. no. C5790 CRITERION™ GC Agar Base 72gm
Cat. no. C5791 CRITERION™ GC Agar Base 500gm
Cat. no. C5792 CRITERION™ GC Agar Base 2kg
Cat. no. C5793 CRITERION™ GC Agar Base 10kg
Cat. no. C5794 CRITERION™ GC Agar Base 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ GC Agar Base is used in the preparation of media for the cultivation and selective isolation of fastidious organisms such as Neisseria and Haemophilus species.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Chocolate, Thayer Martin, Modified Thayer Martin, and Martin Lewis Agars are all used in the cultivation and isolation of Neisseria and Haemophilus species. All four media are formulated by adding supplements to CRITERION™ GC Agar Base.

Hemoglobin and coenzyme enrichments provide growth factors necessary for the cultivation of Haemophilus spp. Antibiotics may also be added to inhibit growth of normal flora microorganisms.

FORMULA

Gram weight per liter: 36.0gm/L
Pancreatic Digest of Casein 7.5gm
Peptic Digest of Animal Tissue 7.5gm
Sodium Chloride 5.0gm
Dipotassium Phosphate 4.0gm
Corn Starch 1.0gm
Monopotassium Phosphate 1.0gm
Agar 10.0gm

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original off-white.

Store the prepared culture medium at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

Note: To prepare chocolate agar base, see Instructions for Use for Criterion™ Hemoglobin Powder, which is prepared separately and added to GC Agar Base along with necessary supplements and/or antibiotics.

1. Suspend 36.0gm of the dehydrated culture media in 1 liter of distilled or deionized water. To prepare Chocolate Agar, suspend 36.0g of powder in 500ml of distilled or deionized water (500ml of separately autoclaved Hemoglobin Solution will be added after autoclaving for a final volume of 1 liter).

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool to 45-50ºC. and add 2% hemoglobin, enrichments, and selective ingredients as desired.

ENRICHED AND SELECTIVE FORMULAS

Chocolate Agar: CRITERION™ GC Agar Base, 2% hemoglobin, and coenzyme enrichments.

Thayer Martin Agar: CRITERION™ GC Agar Base, 2% hemoglobin, coenzyme enrichments, vancomycin (3.0mg/L), colistin (7.5mg/L), and nystatin (1250units/L).

Modified Thayer Martin Agar: CRITERION™ GC Agar Base, 2% hemoglobin, coenzyme enrichments, vancomycin (3.0mg/L), colistin (7.5mg/L), nystatin (1250units/L), trimethoprim (5.0mg/L), and dextrose (1.5gm/L).

Martin Lewis Agar: CRITERION™ GC Agar Base, 2% hemoglobin, coenzyme enrichments, vancomycin (4.0mg/L), colistin (7.5mg/L), lincomycin (1.0mg/L), trimethoprim (5.0mg/L), and dextrose (1.5gm/L).

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. E14 (Chocolate Agar), E130 (Thayer Martin), E30 (MTM), or E31 (Martin Lewis).

LIMITATIONS

Enrichments including hemoglobin and coenzymes, must be added to CRITERION™ GC Agar Base in order to obtain growth of Neisseria and Haemophilus species.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Typical results with hemoglobin and coenzyme enrichments added.

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Chocolate Agar:
Neisseria gonorrhoeae
ATCC ® 43069
A 24hr 35°C CO 2 ** Growth
Neisseria meningitidis
ATCC ® 13090
A 24hr 35°C CO 2 ** Growth

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ GC Agar Base powder should appear homogeneous, free-flowing, and off-white in color, with or without black specks. The prepared media without hemoglobin should appear opaque, and light to medium amber in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

5. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

6. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

7. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.


ATCC is a registered trademark of the American Type Culture Collection.

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