CRITERION™ Gelatin

Cat. no. C7920 CRITERION™ Gelatin 100gm
Cat. no. C7921 CRITERION™ Gelatin 500gm
Cat. no. C7922 CRITERION™ Gelatin 2kg
Cat. no. C7923 CRITERION™ Gelatin 10kg
Cat. no. C7924 CRITERION™ Gelatin 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Gelatin is recommended for use in the preparation of culture media for microbiological purposes.

This is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY(1)

Gelatin is used in some food products to enhance the texture and cohesiveness of the product as well as an ingredient in solid culture media. It is a protein derived from collagen hydrolysis and generally stiffens or congeals the prepared solution. Collagen is found in skin, bones, tendons and the cartilage of animals. In microbiology, gelatin is commonly used in concentrations of ten to twenty percent in order to solidify media for growing different species of microorganisms. This is not an ideal situation due to a melting point of 28 to 30ºC., which is significantly lower than the optimum temperature to grow bacteria.

Robert Koch was the first to use gelatin in 1875 and discovered the ability of certain bacteria to liquify the solid gelatin at room temperature. The enzymes responsible for this were later discovered to be gelatinases; the purpose of these proteolytic enzymes is to break down large molecules so they can be brought into the cell to be metabolized. The ability to liquify gelatin is characteristic of certain Enterobacteriaceae such as Proteus species and Serratia species.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

PRECAUTIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, test tubes and caps, incinerators, and incubators, etc., are not provided.

Physical Appearance

CRITERION™ Gelatin powder should appear homogeneous, free-flowing, and light beige in color.

REFERENCES

1. Gershenfeld, Louis and Linwood F. Tice. 1941. Gelatin for Bacteriological Use. Journal of Bacteriology; 41: 645.

2. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

3. Howard, B.J., et al. 1997. Clinical and Pathogenic Microbiology, 2nd ed. Mosby, St. Louis, MO.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.


ATCC is a registered trademark of the American Type Culture Collection.

053116gr