CRITERION™ HEART INFUSION AGAR

Cat. no. C5820 CRITERION™ Heart Infusion Agar 80gm
Cat. no. C5821 CRITERION™ Heart Infusion Agar 500gm
Cat. no. C5822 CRITERION™ Heart Infusion Agar 2kg
Cat. no. C5823 CRITERION™ Heart Infusion Agar 10kg
Cat. no. C5824 CRITERION™ Heart Infusion Agar 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Heart Infusion Agar is a general purpose growth medium. It is recommended for the cultivation of nutritionally fastidious microorganisms and as a basal medium with a variety of applications.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

CRITERION™ Heart Infusion Agar can be used as base in various formulations. For instance, carbohydrates can be added Heart Infusion Agar to determine fermentation reactions, animal blood to evaluate hemolytic reactions, while antibiotics or other ingredients can be added for the mass cultivation of microorganisms required in biochemical testing and in the preparation of vaccines.(2,3,6) Heart Infusion Media have also been specified for the isolation of Vibrio cholerae and Vibrio species.(4,5)

A broth medium containing meat infusion was one of the first media used for the cultivation of bacteria. Huntoon prepared a "hormone" broth using fresh beef heart and peptone.(7) He found that the medium, without enrichments, could support the growth of a variety of microorganisms, including nutritionally fastidious organisms, such as, meningococci and pneumococci.(7) Hardy Diagnostics Beef Heart Infusion Media is an improved revision to this original formula. It contains beef heart infusion and peptones, which provide the nitrogen, vitamins, and carbon source to meet the nutritional growth requirements of a variety of organisms. In addition, yeast extract is added to the medium to provide additional vitamins to stimulate organism growth. Sodium chloride is added to maintain osmotic balance in the medium.

FORMULA

Gram weight per liter: 40.0gm/L
Pancreatic Digest of Casein 10.0gm
Peptic Digest of Animal Tissue 5.0gm
Sodium Chloride 5.0gm
Yeast Extract 3.0gm
Beef Heart Infusion 2.0gm
Agar 15.0gm

Final pH 7.4 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 40.0gm of the dehydrated culture media in 1 liter of distilled or deionized water. Stir to mix thoroughly.

2. Heat as necessary to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool to 45-50ºC.

5. Aseptically pour into the desired containers.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. Q59.

LIMITATIONS

Due to the various nutritional requirements of some organisms, occasional isolates may be encountered which fail to grow or grow poorly on this medium.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Escherichia coli
ATCC ® 25922
A 18-48hr 35°C Aerobic Plain: growth
With 5% Sheep Blood: growth; beta-hemolysis
Staphylococcus aureus
ATCC ® 25923
A 18-48hr 35°C Aerobic Plain: growth
With 5% Sheep Blood: growth; beta-hemolysis
Streptococcus pneumoniae
ATCC ® 6305
A 18-48hr 35°C Aerobic Plain: growth
With 5% Sheep Blood: growth; alpha-hemolysis
Streptococcus pyogenes
ATCC ® 19615
A 18-48hr 35°C Aerobic Plain: growth
With 5% Sheep Blood: growth; beta-hemolysis

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Heart Infusion Agar powder should appear homogeneous, free-flowing, and beige in color. The prepared media should appear clear, and light to medium amber in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Atlas, R.M. 1997. Handbook of Microbiological Media, 2nd ed. CRC Press, Inc., Boca Raton, FL.

4. Vanderzant, C. PhD. and D.F. Splittstoesser, PhD. 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. American Public Health Association, Washington, D.C.

5. U.S. Food and Drug Administration. Bacteriological Analytical Manual. AOAC, Arlington, VA.
http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006949.htm.

6. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

7. Huntoon, F.M. 1918. "Hormone" Medium. A simple medium employable as a substitute for Serum Medium. The Journal of Infectious Disease; 23:169-172.

8. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.


053116gr