CRITERION™ INHIBITORY MOLD AGAR

Cat. no. C5890 CRITERION™ Inhibitory Mold Agar 72gm
Cat. no. C5891 CRITERION™ Inhibitory Mold Agar 500gm
Cat. no. C5892 CRITERION™ Inhibitory Mold Agar 2kg
Cat. no. C5893 CRITERION™ Inhibitory Mold Agar 10kg
Cat. no. C5894 CRITERION™ Inhibitory Mold Agar 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Inhibitory Mold Agar is recommended for the selective isolation of fungi.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Inhibitory Mold Agar was formulated by Ulrich for use as a general cultivation medium for various strains of pathogenic fungi.(8) The medium is composed of nutritional factors and inorganic salts which support the growth of most pathogenic fungi. Casein and animal tissue provide growth nutrients. Yeast extract provides a rich source of vitamins. Dextrose, starch and dextrin serve as energy sources. Essential minerals and ions are supplied by sodium chloride and metallic salts. Gram-positive and gram-negative bacteria are inhibited by chloramphenicol, a broad spectrum antimicrobic.

FORMULA

Gram weight per liter: 36.0gm/L
Yeast Extract 5.0gm
Dextrose 5.0gm
Pancreatic Digest of Casein 3.0gm
Peptic Digest of Animal Tissue 2.0gm
Starch 2.0gm
Sodium Phosphate 2.0gm
Dextrin 1.0gm
Magnesium Sulfate 0.8gm
Manganese Sulfate 0.16gm
Chloramphenicol 0.125gm
Sodium Chloride 0.04gm
Agar 15.0gm

Final pH 6.7 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared plated media at 2-8ºC. The prepared tubed and bottled media may be stored at 2-30ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 36.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool to 50-55ºC. and dispense.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. W25.

LIMITATIONS

For proper identification of fungi, microscopic examination and evaluation of morphological structures is required. Further biochemical, physiological, serological tests and microscopic morphology of pure cultures are recommended for complete identification. For more information see appropriate references.

Specific strains of fungi for which the medium is designed to isolate often may be inhibited. Fungi for which the medium is designed to inhibit may grow.

A non-selective and selective medium should be inoculated for isolation of fungi from potentially contaminated specimens.

Due to the incorporation of chloramphenicol, the medium is not recommended for use in culturing sterile body fluids.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Trichophyton mentagrophytes
ATCC ® 9533
G 7 days 15-30°C Aerobic Growth
Candida albicans
ATCC ® 10231
A 48hr 35°C Aerobic Growth
Escherichia coli
ATCC ® 25922
B 24hr 35°C Aerobic Partial to complete inhibition

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Inhibitory Mold Agar powder should appear homogeneous, free-flowing, and light tan in color. The prepared media should appear opalescent, and light amber in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Cumitech 11: Practical Methods for Culture and Identification of Fungi in the Clinical Microbiology Laboratory. 1980. American Society for Microbiology, Washington, D.C.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

6. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

7. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

8. Ulrich. 1956. Bacteriol. Proc., S.A.B.; M75, p. 87.

9. St. Germain, Guy, et al. 1996. Identifying Filamentous Fungi. Star Publishing Company, Belmont, CA.


ATCC is a registered trademark of the American Type Culture Collection.

053116gr