CRITERION™ LACTOBACILLI MRS BROTH

Cat. no. C5930 CRITERION™ Lactobacilli MRS Broth 110gm
Cat. no. C5931 CRITERION™ Lactobacilli MRS Broth 500gm
Cat. no. C5932 CRITERION™ Lactobacilli MRS Broth 2kg
Cat. no. C5933 CRITERION™ Lactobacilli MRS Broth 10kg
Cat. no. C5934 CRITERION™ Lactobacilli MRS Broth 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Lactobacilli MRS Broth is used for the isolation, enumeration and cultivation of Lactobacillus spp.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

MRS Broth was developed by deMan, et al. to support luxuriant growth of all lactobacilli from oral, fecal, dairy and other sources.(6) Lactobacilli MRS Broth is an improved medium for lactobacilli, it supports good growth and is particularly useful for a number of fastidious strains which grow poorly on other general media. In addition, tomato juice is not required. In a slightly modified form, it can also be used as a basal medium for fermentation tests.

Lactobacilli MRS Broth contains peptones and dextrose which supply nitrogen and carbon. Tween® 80, acetate, magnesium and manganese provide growth factors for culturing a variety of lactobacilli. These ingredients may inhibit the growth of some organisms other than lactobacilli.

FORMULA

Gram weight per liter: 55.0gm/L
Dextrose 20.0gm
Peptic Digest of Animal Tissue 10.0gm
Beef Extract 10.0gm
Yeast Extract 5.0gm
Sodium Acetate 5.0gm
Disodium Phosphate 2.0gm
Ammonium Citrate 2.0gm
Tween ® 80 1.0gm
Magnesium Sulfate 0.1gm
Manganese Sulfate 0.05gm

Final pH 6.5 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-8ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not homogeneous, moist, and lumpy or if the color has changed from its original tan.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 55.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Dispense into tubes or containers as desired. Durham tubes can be added when testing for gas production.

4. Sterilize in the autoclave at 121ºC. for 15 minutes.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. K15.

LIMITATIONS

Organisms other than lactobacilli may grow in this media.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Weisella paramesenteroides
ATCC ® 33313
A 1-7 days 35°C Aerobic Growth; gas positive
Lactobacillus acidophilus
ATCC ® 4356
A 1-7 days 35°C Aerobic Growth; gas negative

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Lactobacilli MRS Broth powder should appear homogeneous, moist, and lumpy, and tan in color. The prepared media should appear clear, with a slight opalescence, and dark amber in color.

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

4. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

5. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

6. deMan, Rogosa and Sharpe. 1960. Journal of Applied Bacteriology; 23(1) 130-135.


ATCC is a registered trademark of the American Type Culture Collection.
Tween is a registered trademark of ICI Americas, Inc.

053116gr