CRITERION™ LISTERIA ENRICHMENT BROTH

Cat. no. C6030 CRITERION™ Listeria Enrichment Broth 72.2gm
Cat. no. C6031 CRITERION™ Listeria Enrichment Broth 500gm
Cat. no. C6032 CRITERION™ Listeria Enrichment Broth 2kg
Cat. no. C6033 CRITERION™ Listeria Enrichment Broth 10kg
Cat. no. C6034 CRITERION™ Listeria Enrichment Broth 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Listeria Enrichment Broth is used for the selective enrichment of Listeria monocytogenes.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Listeria Enrichment Broth is used in procedures for the isolation of Listeria monocytogenes from clinical and environmental samples.(1,8) Isolation procedures for Listeria monocytogenes typically involve one or more enrichment steps prior to plating on differential media.

Listeria Enrichment Broth contains peptones and dextrose as a source of growth factors. Phosphate salts maintain the proper pH of the media. Cycloheximide is incorporated to inhibit saprophytic fungi. The selective agents, nalidixic acid, and acriflavine act to inhibit gram-negative and gram-positive organisms, respectively.

FORMULA

Gram weight per liter: 36.1gm/L
Pancreatic Digest of Casein 17.0gm
Yeast Extract 6.0gm
Sodium Chloride 5.0gm
Papaic Digest of Soybean Meal 3.0gm
Dextrose 2.5gm
Dipotassium Phosphate 2.5gm
Cycloheximide 50.0mg
Nalidixic Acid 40.0mg
Acriflavine 15.0mg

Final pH 7.3 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 36.1gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. U93.

LIMITATIONS

Listeria Enrichment Broth is intended to aid in the isolation and identification of Listeria monocytogenes . Additional biochemical and/or serological testing may be required for complete identification, consult listed references for more information.(1,2,4)

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Listeria monocytogenes
ATCC ® 7644
A 24-48hr 35°C Aerobic Growth
Escherichia coli
ATCC ® 25922
B 24-48hr 35°C Aerobic Partial to complete inhibition
Staphylococcus aureus
ATCC ® 25923
B 24-48hr 35°C Aerobic Partial to complete inhibition

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Listeria Enrichment Broth powder should appear homogeneous, free-flowing, and light beige in color. The prepared media should appear clear, and light amber in color.

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

4. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

5. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

6. FDA. 1995. Bacteriological Analytical Manual, 8th ed. FDA.

7. Marshall, R.T., ed. 1992. Standard Methods for the Examination of Dairy Products, 16th ed. APHA, Washington, D.C.

8. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.

9. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.


ATCC is a registered trademark of the American Type Culture Collection.

060616gr