CRITERION™ MODIFIED BUFFERED PEPTONE WATER

Cat. no. C6350 CRITERION™ Modified Buffered Peptone Water 50gm
Cat. no. C6351 CRITERION™ Modified Buffered Peptone Water 500gm
Cat. no. C6352 CRITERION™ Modified Buffered Peptone Water 2kg
Cat. no. C6353 CRITERION™ Modified Buffered Peptone Water 10kg
Cat. no. C6354 CRITERION™ Modified Buffered Peptone Water 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Modified Buffered Peptone Water is used for preenriching Salmonella species from food specimens to increase recovery.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Salmonella spp. are prone to injury from many food preservation techniques involving heat, high osmotic pressure or pH, or preservatives. Modified Buffered Peptone Water facilitates the recovery of Salmonellae. Modified Buffered Peptone Water provides additional buffering capacity when organisms have been enriched in a preenrichment medium containing a high carbohydrate concentration.

Modified Buffered Peptone Water contains peptone as a source of carbon, nitrogen, vitamins and minerals. Sodium chloride maintains the osmotic balance. Phosphates buffer the medium.

FORMULA

Gram weight per liter: 25gm/L
Peptone 10.0gm
Disodium Phosphate 7.0gm
Sodium Chloride 5.0gm
Monopotassium Phosphate 3.0gm

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared culture medium at 2-30ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 25.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclave, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Salmonella enterica
ATCC ® 14028
A 18-24hr 35°C Aerobic Growth when subcultured to XLD
Escherichia coli
ATCC ® 25922
A 18-24hr 35°C Aerobic Partial to complete inhibition when subcultured to XLD
Proteus hauseri
ATCC ® 13315
B 18-24hr 35°C Aerobic Partial to complete inhibition when subcultured to XLD

User Quality Control

REFERENCES

1. FDA. 1995. Bacteriological Analytical Manual, 8th ed. FDA.

2. Marshall, R.T., ed. 1992. Standard Methods for the Examination of Dairy Products, 16th ed., APHA, Washington, D.C.

3. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.

4. Sadovski, A.Y. 1977. J. Food Technology; 12: 85-91.

5. Juven, B.J, N. Cox, J.S. Bailey, J.E. Thomson, O.W. Charles, and J. V. Schutze. 1984. Recovery of Salmonella from artificially contaminated poultry feeds in non-selective and selective broth media. Jour. of Food Prot.; 47:299-302.


ATCC is a registered trademark of the American Type Culture Collection.

060616gr