CRITERION™ NUTRIENT AGAR

Cat. no. C6460 CRITERION™ Nutrient Agar 41gm
Cat. no. C6461 CRITERION™ Nutrient Agar 500gm
Cat. no. C6462 CRITERION™ Nutrient Agar 2kg
Cat. no. C6463 CRITERION™ Nutrient Agar 10kg
Cat. no. C6464 CRITERION™ Nutrient Agar 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Nutrient Agar is a general purpose growth media recommended for use in the isolation and cultivation of nonfastidious microorganisms.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

The formulation of CRITERION™ Nutrient Agar is composed of peptone and beef extract. Nutrients necessary for the replication and growth of a large number of nonfastidious microorganisms are provided by the simple formulation. Water soluble substances including carbohydrates, vitamins, organic nitrogen compounds and salts are present in the beef extract.(9) Pancreatic Digest of Gelatin supplies the principle source of organic nitrogen in the form of amino acids and long-chained fatty acids.

FORMULA

Gram weight per liter: 20.5gm/L
Pancreatic Digest of Gelatin 5.0gm
Beef Extract 3.0gm
Agar 12.5gm

Final pH 6.8 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 20.5gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. W51.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerator, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Staphylococcus epidermidis
ATCC ® 12228
A 18-24hr 35°C Aerobic Growth
Escherichia coli
ATCC ® 25922
A 18-24hr 35°C Aerobic Growth

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Nutrient Agar powder should appear homogeneous, free-flowing, and beige in color. The prepared media should appear slightly opalescent, and light amber in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Greenberg, A.E., et al. (ed.). 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. APHA, Washington, D.C.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

6. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

7. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

8. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.

9. Pelczar, Chan and Kreig, Microbiology, 5th ed. 1986. McGraw-Hill Book Company, New York.


ATCC is a registered trademark of the American Type Culture Collection.

061416gr