CRITERION™ PEPTONE WATER

Cat. no. C6570 CRITERION™ Peptone Water 30gm
Cat. no. C6571 CRITERION™ Peptone Water 500gm
Cat. no. C6572 CRITERION™ Peptone Water 2kg
Cat. no. C6573 CRITERION™ Peptone Water 10kg
Cat. no. C6574 CRITERION™ Peptone Water 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Peptone Water is used for the cultivation of nonfastidious organisms, for performing the indole test, and for studying fermentation patterns.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Peptone Water is useful for the cultivation of non-fastidious organisms. (1) Peptone in the medium supplies nutrients necessary for the growth of those microorganisms. Peptone Water is a minimal media, unsuitable for the growth of fastidious microorganisms, but ideal for use as a basal medium for biochemical tests such as carbohydrate fermentation and indole production.(1,2)

CRITERION™ Peptone Water contains peptone as a source of growth nutrients including carbon, nitrogen, vitamins and minerals. Sodium chloride maintains osmotic balance.

FORMULA

Gram weight per liter: 15.0gm/L
Peptone 10.0gm
Sodium Chloride 5.0gm

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light tan.

Store the prepared culture media at 2-30ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 15.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to and mix to dissolve completely.

3. Autoclave at 121ºC. for 15 minutes.

For Performing Carbohydrate Fermentation

1. Suspend 15.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat and mix to dissolve completely.

3. Add 1.8ml 1% phenol red solution to 1 liter of Peptone Water.

4. Dispense into tubes containing inverted durham tubes.

5. Sterilize in the autoclave at 121ºC. for 15 minutes.

6. Aseptically add sufficient sterile carbohydrate solution to yield a 1% final concentration. Rotate each tube to thoroughly distribute the carbohydrate.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. K47.

LIMITATIONS

Vibrio spp. should not be incubated longer than 18-20 hours. Longer incubation may cause development of suppressed forms. (3)

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Carbohydrate Fermentation (Peptone Water with added phenol red and carbohydrate)
Escherichia coli
ATCC ® 25922
E 18-24hr 35°C Aerobic Growth; media turns yellow, gas positive
Moraxella catarrhalis
ATCC ® 25240
E 18-24hr 35°C Aerobic Growth; no color change, gas negative
Growth/Indole Reaction
Escherichia coli
ATCC ® 25922
E 18-24hr 35°C Aerobic Growth; turns pink to red after adding 4-5 drops of Kovacs Reagent and agitation
Staphylococcus aureus
ATCC ® 25923
E 18-24hr 35°C Aerobic Growth; no pink color after adding Kovacs Reagent

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Peptone Water powder should appear homogeneous, free-flowing, and light tan in color. The prepared media should appear clear, and light amber in color.

REFERENCES

1. MacFaddin, J.F. Biochemical Tests for Identification of Medical Bacteria,, Lipincott Williams & Wilkins, Philadelphia, PA.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.


ATCC is a registered trademark of the American Type Culture Collection.

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