CRITERION™ PEPTONE WATER WITH LECITHIN AND TWEEN®

Cat. no. C8180 CRITERION™ Peptone Water with Lecithin and Tween® 34gm
Cat. no. C8181 CRITERION™ Peptone Water with Lecithin and Tween® 500gm
Cat. no. C8182 CRITERION™ Peptone Water with Lecithin and Tween® 2kg
Cat. no. C8183 CRITERION™ Peptone Water with Lecithin and Tween® 10kg
Cat. no. C8184 CRITERION™ Peptone Water with Lecithin and Tween® 50kg

INTENDED USE

Hardy Diagnostics' CRITERION™ Peptone Water with Lecithin and Tween® is recommended for the cultivation of microorganisms from environmental samples and the neutralization of disinfectants.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

CRITERION™ Peptone Water with Lecithin and Tween® is useful as a minimal growth medium for the cultivation of injured or damaged cells from the environment.(4) Peptone in the medium supplies nutrients necessary for cell growth and maintenance. Sodium chloride is added to maintain osmotic balance. Lecithin and Tween® 80 are surfactants added to neutralize the influence of residual antimicrobials and to and aid in the recovery of stressed or injured cells. Residues left by germicidal or disinfecting agents often result in lowered microbial counts; therefore, neutralization of residual disinfectants limits their inhibitory effect. Quaternary ammonia compounds are neutralized by lecithin while phenolic disinfectants and hexachlorophene are neutralized by Tween® 80; together, lecithin and Tween® 80 neutralize ethanol.(1,2)

FORMULA*

Gram weight per liter: 17gm/L
Gelatin Peptone 10.0gm
Sodium Chloride 5.0gm
Lecithin 1.0gm
Tween® 80 1.0gm

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-8ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not moist and lumpy or if the color has changed from its original beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 17.0gm of the dehydrated culture media in one liter of distilled or deionized water. Stir to mix thoroughly.

2. Heat as necessary to dissolve completely.

3. Dispense desired volume into tubes or bottles, leaving head space, and cap loosely.

4. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool the medium to 45-50ºC. and tighten caps for long-term storage.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, prepared media containers, other culture media, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Escherichia coli
ATCC ® 25922
A 18-24hr 35°C Aerobic Growth
Staphylococcus aureus
ATCC ® 25923
A 18-24hr 35°C Aerobic Growth

User Quality Control

REFERENCES

1. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.

2. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

5. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.


ATCC is a registered trademark of the American Type Culture Collection.
Tween is a registered trademark of ICI Americas, Inc.

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