CRITERION™ PHENOL RED BROTH BASE

Cat. no. C6590 CRITERION™ Phenol Red Broth Base 32gm
Cat. no. C6591 CRITERION™ Phenol Red Broth Base 500gm
Cat. no. C6592 CRITERION™ Phenol Red Broth Base 2kg
Cat. no. C6593 CRITERION™ Phenol Red Broth Base 10kg
Cat. no. C6594 CRITERION™ Phenol Red Broth Base 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Phenol Red Broth Base is intended to be used in the differentiation of microorganisms based on fermentation reactions.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Phenol Red Carbohydrate Test Media contains pancreatic digest of casein, sodium chloride, a carbohydrate, and phenol red. Pancreatic digest of casein provides the nutrients and growth factors necessary for growth. This media is low in carbohydrates which may interfere with the fermentation reactions of the media. The individual carbohydrates are added at a concentration of 1%. Phenol red is the acid-base indicator in the media. It is red at an alkaline pH, and shifts to yellow when acid is produced during carbohydrate fermentation. Durham tubes, also known as fermentation tubes, are added to visualize gas production produced during carbohydrate fermentation reactions. After incubation, yellow media with gas trapped in the durham tube is indicative of positive acid and gas fermentation reactions.

FORMULA*

Gram weight per liter: 16.0gm/L
Pancreatic Digest of Casein 10.0gm
Sodium Chloride 5.0gm
Phenol Red 18.0mg

Final pH 7.4 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original pink.

Store the prepared culture medium at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Dissolve 16.0gm in 1 liter of distilled or deionized water.

2. Distribute into tubes. To detect gas production, place inverted fermentation tubes (durham tubes) in the tubes of medium.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

When preparing 0.5-1% carbohydrate fermentation broths, dissolve 5-10gm of the desired carbohydrate in the basal medium prior to sterilization, or dissolve 16.0gm of Phenol Red Broth Base in 900ml distilled or deionized water and aseptically add 100ml of a sterile 5-10% carbohydrate solution after sterilizing and cooling the basal medium.

PROCEDURE

Specimen Collection: This product is not intended for primary isolation of patient specimens. It should be used only with cultures of isolated organism. This product is used in conjunction with other biochemical tests to identify cultures of isolated organism.

Inoculate tubes with one drop of a diluted pure culture.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. Y300.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Dextrose is added at a final concentration of 1% to the tubes of prepared Phenol Red Broth Base before QC testing.

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Phenol Red Base:
Escherichia coli
ATCC ® 25922
A 18-24hr 35°C Aerobic Growth; yellow color change and gas bubble in durham tube
Shigella flexneri
ATCC ® 12022
B 18-24hr 35°C Aerobic Growth; yellow color change, no gas bubble in durham tube

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Phenol Red Broth Base powder should appear homogeneous, free-flowing, and pink in color. The prepared media should appear clear, and red in color.

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

4. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

5. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.


ATCC is a registered trademark of the American Type Culture Collection.

062016gr