CRITERION™ PHENOL RED BROTH BASE
|Cat. no. C6590||CRITERION™ Phenol Red Broth Base||32gm|
|Cat. no. C6591||CRITERION™ Phenol Red Broth Base||500gm|
|Cat. no. C6592||CRITERION™ Phenol Red Broth Base||2kg|
|Cat. no. C6593||CRITERION™ Phenol Red Broth Base||10kg|
|Cat. no. C6594||CRITERION™ Phenol Red Broth Base||50kg|
Hardy Diagnostics CRITERION™ Phenol Red Broth Base is intended to be used in the differentiation of microorganisms based on fermentation reactions.
This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.
Phenol Red Carbohydrate Test Media contains pancreatic digest of casein, sodium chloride, a carbohydrate, and phenol red. Pancreatic digest of casein provides the nutrients and growth factors necessary for growth. This media is low in carbohydrates which may interfere with the fermentation reactions of the media. The individual carbohydrates are added at a concentration of 1%. Phenol red is the acid-base indicator in the media. It is red at an alkaline pH, and shifts to yellow when acid is produced during carbohydrate fermentation. Durham tubes, also known as fermentation tubes, are added to visualize gas production produced during carbohydrate fermentation reactions. After incubation, yellow media with gas trapped in the durham tube is indicative of positive acid and gas fermentation reactions.
|Gram weight per liter:||16.0gm/L|
|Pancreatic Digest of Casein||10.0gm|
Final pH 7.4 +/- 0.2 at 25ºC.
* Adjusted and/or supplemented as required to meet performance criteria.
STORAGE AND SHELF LIFE
Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original pink.
Store the prepared culture medium at 2-8ºC.
METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA
1. Dissolve 16.0gm in 1 liter of distilled or deionized water.
2. Distribute into tubes. To detect gas production, place inverted fermentation tubes (durham tubes) in the tubes of medium.
3. Sterilize in the autoclave at 121ºC. for 15 minutes.
When preparing 0.5-1% carbohydrate fermentation broths, dissolve 5-10gm of the desired carbohydrate in the basal medium prior to sterilization, or dissolve 16.0gm of Phenol Red Broth Base in 900ml distilled or deionized water and aseptically add 100ml of a sterile 5-10% carbohydrate solution after sterilizing and cooling the basal medium.
Specimen Collection: This product is not intended for primary isolation of patient specimens. It should be used only with cultures of isolated organism. This product is used in conjunction with other biochemical tests to identify cultures of isolated organism.
Inoculate tubes with one drop of a diluted pure culture.
PROCEDURE AND INTERPRETATION OF RESULTS
For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. Y300.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.
Dextrose is added at a final concentration of 1% to the tubes of prepared Phenol Red Broth Base before QC testing.
|Test Organisms||Inoculation Method*||Incubation||Results|
|Phenol Red Base:|
ATCC ® 25922
|A||18-24hr||35°C||Aerobic||Growth; yellow color change and gas bubble in durham tube|
ATCC ® 12022
|B||18-24hr||35°C||Aerobic||Growth; yellow color change, no gas bubble in durham tube|
User Quality Control
CRITERION™ Phenol Red Broth Base powder should appear homogeneous, free-flowing, and pink in color. The prepared media should appear clear, and red in color.
1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.
2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.
3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.
4. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.
5. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.
ATCC is a registered trademark of the American Type Culture Collection.