CRITERION™ RAPPAPORT-VASSILIADIS R10 BROTH

Cat. no. C6730 CRITERION™ Rappaport-Vassiliadis R10 Broth 53.2gm
Cat. no. C6731 CRITERION™ Rappaport-Vassiliadis R10 Broth 500gm
Cat. no. C6732 CRITERION™ Rappaport-Vassiliadis R10 Broth 2kg
Cat. no. C6733 CRITERION™ Rappaport-Vassiliadis R10 Broth 10kg
Cat. no. C6734 CRITERION™ Rappaport-Vassiliadis R10 Broth 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Rappaport-Vassiliadis R10 Broth is recommended for the selective enrichment of Salmonella spp. from food and environmental specimens.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Rappaport medium was initially developed by Rappaport, et al., in 1956 as an alternative to Tetrathionate Broth for the enrichment of Salmonella.(1) A high concentration of magnesium chloride was included to inhibit growth of Proteus spp. and Escherichia coli. The addition of malachite green inhibits coliforms. The low pH also inhibits microorganisms other that Salmonella. In 1976, Vassiliadis, et al., described a modification of Rappaport medium called R10. This formula featured a reduced amount of malachite green.(2) CRITERION™ Rappaport-Vassiliadis R10 Broth is based on this formula.

FORMULA*

Gram weight per liter: 26.6gm/L
Magnesium Chloride 13.4gm
Sodium Chloride 7.2gm
Pancreatic Digest of Casein 4.54gm
Potassium Phosphate 1.45gm
Malachite Green 36.0mg

Final pH 5.1 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original pale green.

Store the prepared culture media at 2-30ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 26.6gm of the dehydrated culture media in 1 liter of distilled or deionized water. Stir to mix thoroughly.

2. Heat as necessary to dissolve completely. Do not overheat.

3. Sterilize in the autoclave at 116ºC. for 15 minutes.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. K167.

LIMITATIONS

Rappaport-Vassiliadis R10 Broth is a selective enrichment for Salmonella. Other biochemical and/or serological tests must be performed for complete identification. See listed references.(3,5,6,8-10)

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Salmonella enterica
ATCC ® 14028
A 18-24hr 35°C Aerobic Growth when subcultured to XLD Agar
Escherichia coli
ATCC ® 25922
B 18-24hr 35°C Aerobic Inhibited when subcultured to XLD Agar
Proteus hauseri
ATCC ® 13315
B 18-24hr 35°C Aerobic Inhibited when subcultured to XLD Agar

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Rappaport-Vassiliadis R10 Broth powder should appear homogeneous, free-flowing, and pale green in color. The prepared media should appear clear, and blue-green in color.

REFERENCES

1. Rappaport, F., et al. 1956. J. Clin. Path.; 9:261-266.

2. Vassiliadis, P., et al. 1978. J. Appl. Bact.; 44:233-239.

3. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

4. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

6. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

7. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

8. FDA. 1995. Bacteriological Analytical Manual, 8th ed. FDA.

9. Marshall, R.T., ed. 1992. Standard Methods for the Examination of Dairy Products, 16th ed. APHA, Washington, D.C.

10. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.

11. Greenberg, A.E., et al. (ed.). 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. APHA, Washington, D.C.

12. U.S. Pharmacopeia, 22nd rev. 1990. U.S. Pharmacopeial Convention, Rockville, MD.

13. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.


ATCC is a registered trademark of the American Type Culture Collection.

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