CRITERION™ SABOURAUD DEXTROSE (SABDEX) AGAR WITH LECITHIN AND TWEEN® 80

Cat. no. C6800 CRITERION™ SabDex Agar with Lecithin and Tween® 80 138gm
Cat. no. C6801 CRITERION™ SabDex Agar with Lecithin and Tween® 80 500gm
Cat. no. C6802 CRITERION™ SabDex Agar with Lecithin and Tween® 80 2kg
Cat. no. C6803 CRITERION™ SabDex Agar with Lecithin and Tween® 80 10kg
Cat. no. C6804 CRITERION™ SabDex Agar with Lecithin and Tween® 80 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Sabouraud Dextrose Agar with Lecithin and Tween® 80 is recommended for the cultivation of fungi from environmental surfaces.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Sabouraud Dextrose (Sabdex) Agar was formulated by Sabouraud in 1892, for culturing dermatophytes.(5) The pH is adjusted to approximately 5.6 in order to enhance the growth of fungi, especially dermatophytes, and to slightly inhibit bacterial growth in clinical specimens.(2) This medium is recommended for mold and yeast counts by the U.S. Pharmacopeia, Standard Methods for the Examination of Water and Wastewater, the Association of Official Analytical Chemists, and the Compendium of Methods for the Microbiological Examination of Foods.(1,2,6,7)

Germicidal or disinfectant residue from environmental surfaces is neutralized by the addition of lecithin and Tween® 80. Neutralization of these residues reduces their inhibitory effect which would ultimately result in the lowering of the microbial count. Quaternary ammonia compounds are neutralized by lecithin while phenolic disinfectants and hexachlorophene are neutralized by Tween® 80. Together, lecithin and Tween® 80 neutralize ethanol.

Sabouraud Dextrose Medium contains digests of animal tissues (peptones) which provide a source of amino acids and nitrogenous compounds for the growth of fungi and yeasts. Dextrose is added as the energy and carbon source. Chloramphenicol may be added as a broad spectrum antimicrobial, to inhibit growth of a wide range of gram-positive and gram-negative bacteria.

FORMULA

Gram weight per liter: 71.0gm/L
Dextrose 40.0gm
Pancreatic Digest of Casein 10.0gm
Tween® 80 5.0gm
Lecithin 0.7gm
Agar 15.0gm

Final pH 5.6 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-8ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not homogeneous, moist, and lumpy or if the color has changed from its original light beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 69.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes. Do not overheat.

4. Cool to 45-50ºC.

To prepare contact plates, aseptically pour approximately 17ml into 15x60mm plates to give a meniscus of agar which extends above the top of the poured plate.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. W71.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Aspergillus brasiliensis
formerly A. niger
ATCC ® 16404

J up to 7 days 15-30°C Aerobic Growth
Candida albicans
ATCC ® 10231
J up to 7 days 15-30°C Aerobic Growth
Trichophyton mentagrophytes
ATCC ® 9533
J up to 7 days 15-30°C Aerobic Growth, may take up to one week

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Sabouraud Dextrose Agar with Lecithin and Tween® 80 powder should appear homogeneous, moist, and lumpy, and light beige in color. The prepared media should appear slightly opalescent, and light amber in color.

REFERENCES

1. Association of Official Analytical Chemists. Official Methods of Analysissm, AOAC, Washington, D.C.

2. Greenberg, A.E., et al. (ed.). 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. APHA, Washington, D.C.

3. Larone, D.H. Medically Important Fungi: A Guide to Identification, American Society for Microbiology. Washington, D.C.

4. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

5. Sabouraud, R. 1892. Ann. Dermatol. Syphil.; 3:1061.

6. U.S. Pharmacopeia, 22nd rev. 1990. U.S. Pharmacopeial Convention, Rockville, MD.

7. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.


ATCC is a registered trademark of the American Type Culture Collection.
Tween is a registered trademark of ICI Americas, Inc.

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