CRITERION™ SABOURAUD DEXTROSE (SabDex) AGAR WITH CHLORAMPHENICOL

Cat. no. C6780 CRITERION™ SabDex Agar with Chloramphenicol 127gm
Cat. no. C6781 CRITERION™ SabDex Agar with Chloramphenicol 500gm
Cat. no. C6782 CRITERION™ SabDex Agar with Chloramphenicol 2kg
Cat. no. C6783 CRITERION™ SabDex Agar with Chloramphenicol 10kg
Cat. no. C6784 CRITERION™ SabDex Agar with Chloramphenicol 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Sabouraud Dextrose (SabDex) Agar with Chloramphenicol is recommended as a selective agar medium for the isolation, identification and cultivation of fungi.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Sabouraud Dextrose Agar was formulated by Sabouraud in 1892, for culturing dermatophytes.(13) The pH is adjusted to approximately 5.6 in order to enhance the growth of fungi, especially dermatophytes, and to slightly inhibit bacterial growth in clinical specimens.(2) Sabouraud Dextrose Agar is recommended for mold and yeast counts by the U.S. Pharmacopeia, Standard Methods for the Examination of Water and Wastewater, the Association of Official Analytical Chemists, and the Compendium of Methods for the Microbiological Examination of Foods.(3,6,14,15) Chloramphenicol in Sabouraud Dextrose with Chloramphenicol is added to inhibit bacterial overgrowth while permitting successful selective isolation of fungi and yeasts.

Sabouraud Dextrose Medium contains peptones which provide a nutritious source of amino acids and nitrogenous compounds for the growth of fungi and yeasts. Dextrose is added as the energy and carbon source. Chloramphenicol may be added as a broad spectrum antimicrobial, to inhibit growth of a wide range of gram-positive and gram-negative bacteria.

FORMULA

Gram weight per liter: 63.4gm/L
Dextrose 40.0gm
Pancreatic Digest of Casein 5.0gm
Peptic Digest of Animal Tissue 5.0gm
Chloramphenicol 50.0mg
Agar 13.0gm

Final pH 5.6 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 63.4gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC for 15 minutes.

4. Dispense into sterile containers as desired.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. W72.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc. are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Candida albicans
ATCC ® 10231
A 48hr 35°C Aerobic Growth
Trichophyton mentagrophytes
ATCC ® 9533
G 7 days 15-30°C Aerobic Growth
Aspergillus brasiliensis
formerly A. niger
ATCC ® 16404

G 7 days 15-30°C Aerobic Growth
Escherichia coli
ATCC ® 25922
B 24hr 35°C Aerobic Partial to complete inhibition

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ SabDex Agar with Chloramphenicol powder should appear homogeneous, free-flowing, and light beige in color. The prepared media should appear clear to trace hazy, and amber in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Ajello, et al. 1963. CDC Laboratory Manual for Medical Mycology, PHS Publication No. 994. U.S. Gov't Printing Office, Washington, D.C.

3. Association of Official Analytical Chemists (AOAC). 1992. U.S. Food and Drug Administration, Bacteriological Analytical Manual, 7th ed. Arlington, VA.

4. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

5. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

6. Greenberg, A.E., et al. (ed.). 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. APHA, Washington, D.C.

7. Haley, L.D., et al. 1980. Cumitech 11; Practical Methods for Culture and Identification of Fungi in the Clinical Microbiology Laboratory, Coordinating ed., J.C. Sherris. American Society for Microbiology, Washington, D.C.

8. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

9. Kwon-Chung, K.J. and J.E. Bennett. 1992. Medical Mycology. Lea and Febiger, Malvern, PA.

10. Larone, D.H. Medically Important Fungi: A Guide to Identification, American Society for Microbiology. Washington, D.C.

11. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

12. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

13. Sabouraud, R. 1892. Ann. Dermatol. Syphil.; 3:1061.

14. U.S. Pharmacopeia , 23nd rev. 1995. U.S. Pharmacopeial Convention, Rockville, MD.

15. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.


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