CRITERION™ SABOURAUD DEXTROSE (SABDEX) AGAR, EMMONS

Cat. no. C6770 CRITERION™ SabDex Agar, Emmons 94.8gm
Cat. no. C6771 CRITERION™ SabDex Agar, Emmons 500gm
Cat. no. C6772 CRITERION™ SabDex Agar, Emmons 2kg
Cat. no. C6773 CRITERION™ SabDex Agar, Emmons 10kg
Cat. no. C6774 CRITERION™ SabDex Agar, Emmons 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Sabouraud Dextrose Agar, Emmons is recommended for the isolation, cultivation, and maintenance of fungi and yeasts.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Sabouraud Dextrose Agar was formulated by Sabouraud in 1892, for culturing dermatophytes.(13) The pH is adjusted to approximately 5.6 in order to enhance the growth of fungi, especially dermatophytes, and to slightly inhibit bacterial growth in clinical specimens.(2) This medium is recommended for mold and yeast counts by the U.S. Pharmacopeia, Standard Methods for the Examination of Water and Wastewater, the Association of Official Analytical Chemists, and the Compendium of Methods for the Microbiological Examination of Foods.(3,6,14,15) Emmons Sabouraud Dextrose Agar is a modification of the original formulation. Emmons originally formulated this modification, which reduces the amount of dextrose, and neutralizes the medium to a pH of approximately 7.0.(9)

Sabouraud Dextrose Agar, Emmons contains peptones which provide a source of amino acids and nitrogenous compounds for the growth of fungi and yeasts. Dextrose is added as the energy and carbon source. Chloramphenicol may be added as a broad spectrum antimicrobial, to inhibit growth of a wide range of gram-positive and gram-negative bacteria.

FORMULA

Gram weight per liter: 48.4gm/L
Dextrose 20.0gm
Pancreatic Digest of Casein 5.0gm
Peptic Digest of Animal Tissue 5.0gm
Agar 18.4gm

Final pH 6.9 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 47.4gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes. Do not overheat.

4. Cool to 45-50ºC.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. W20.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Candida albicans
ATCC® 10231
J 24-48hr 15-30°C Aerobic Growth
Trichophyton mentagrophytes
ATCC® 9533
J up to 7 days 15-30°C Aerobic Growth, may take up to one week

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Sabouraud Dextrose Agar, Emmons powder should appear homogeneous, free-flowing, and light beige in color. The prepared media should appear translucent, and light amber in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Ajello, et al. 1963. CDC Laboratory Manual for Medical Mycology, PHS Publication No. 994. U.S. Gov't Printing Office, Washington, D.C.

3. Association of Official Analytical Chemists. Official Methods of Analysissm, AOAC, Washington, D.C.

4. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

5. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

6. Greenberg, A.E., et al. (ed.). 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. APHA, Washington, D.C.

7. Haley, L.D., et al. 1980. Cumitech 11; Practical Methods for Culture and Identification of Fungi in the Clinical Microbiology Laboratory, Coordinating ed., J.C. Sherris. American Society for Microbiology, Washington, D.C.

8. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

9. Kwon-Chung, K.J. and J.E. Bennett. 1992. Medical Mycology. Lea and Febiger, Malvern, PA.

10. Larone, D.H. Medically Important Fungi: A Guide to Identification, American Society for Microbiology. Washington, D.C.

11. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

12. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

13. Sabouraud, R. 1892. Ann. Dermatol. Syphil.; 3:1061.

14. U. S. Pharmacopeia, 22nd rev. 1990. U.S. Pharmacopeial Convention, Rockville, MD.

15. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.


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