CRITERION™ Shigella Broth BASE

Cat. no. C9150 CRITERION™ Shigella Broth Base 120gm
Cat. no. C9151 CRITERION™ Shigella Broth Base 500gm
Cat. no. C9152 CRITERION™ Shigella Broth Base 2kg
Cat. no. C9153 CRITERION™ Shigella Broth Base 10kg
Cat. no. C9154 CRITERION™ Shigella Broth Base 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Shigella Broth Base is intended for us with Novobiocin NaCl supplement (Cat. no. Z915A), for the selective enrichment broth used for the isolation of Shigella spp. from food.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Shigella are gram negative, rod shaped, enteric bacteria that are well documented as a food pathogen. Upon consumption, many strains are capable of producing Shiga toxin and enterotoxin, which cause a type of dysentary known as shigellosis. Shigella Broth as described in BAM and APHA is used for the isolation and enrichment of Shigella spp. from food samples.(1,2)

The addition of Novobiocin NaCl supplement (Cat. no. Z915A) to the broth during preparation allows for the inhibition of gram-positive organisms while allowing enteric bacteria such as Shigella to be selectively enriched.

FORMULA

Gram weight per liter: 31.5gm/L
Tryptone 20.0gm
Sodium Chloride 5.0gm
Glucose 1.0gm
Monopotassium Phosphate 2.0gm
Dipotassium Phosphate 2.0gm
Polysorbate 80 1.5ml

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light yellowish-beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 31.4gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool to 45-50ºC. and, if desired, aseptically add 0.5ml pre-sterilized Novobiocin NaCl supplement (Cat. no. Z915A) into the broth.

5. Mix thoroughly before dispensing desired volume into pre-sterilized containers..

PROCEDURE AND INTERPRETATION OF RESULTS

For the enrichement of Shigella spp. from food samples, please consult the listed references.(1,2)

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, incubators, and Novobiocin NaCl supplement, etc. are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Shigella flexneri
ATCC ® 12022
J 18-24hr 35°C Aerobic Growth
Shigella sonnei
ATCC ® 9290
J 18-24hr 35°C Aerobic Growth
Staphylococcus aureus
ATCC ® 25923
J 18-24hr 35°C Aerobic Complete Inhibition***

***When broth is prepared with the Novobiocin NaCl supplement.

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Shigella Broth Base powder should appear homogeneous, free-flowing, and light yellow-beige in color. The prepared media should appear transparent and light amber in color.

REFERENCES

1. U.S. Food and Drug Administration. Bacteriological Analytical Manual. AOAC, Arlington, VA.
http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006949.htm.

2. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.

3. Atlas R.M., 2010, Handbook of Microbiological Media 4th Edition, CRC Press, Boca Raton, New York, London, Tokyo

4. Hale T. L., Keusch G. T., 1996, Shigella. In: Barons Medical Microbiology (Barron S et al, Eds.), 4th Ed., Univ of Texas Medical Branch.


ATCC is a registered trademark of the American Type Culture Collection.

062116gr