CRITERION™ STARCH AGAR

Cat. no. C7010 CRITERION™ Starch Agar 50gm
Cat. no. C7011 CRITERION™ Starch Agar 500gm
Cat. no. C7012 CRITERION™ Starch Agar 2kg
Cat. no. C7013 CRITERION™ Starch Agar 10kg
Cat. no. C7014 CRITERION™ Starch Agar 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Starch Agar is used for cultivating microorganisms being tested for starch hydrolysis.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Starch Agar was originally formulated in order to cultivate Neisseria . Superior media have been developed for the isolation of Neisseria spp. since the original Starch Agar formulation was created in 1915. Currently, Starch Agar Medium for Pseudomonas and Starch Agar with Bromcresol Purple are modifications of Starch Agar used for maintenance of Pseudomonas cultures.

Beef extract provides the nitrogen, vitamins, carbon and amino acids in Starch Agar. Starch Agar reacts with Gram's Iodine to give a blue color. Organisms hydrolyzing starch through amylase production will produce a clearing around the isolate while the remaining medium is blue. Agar is a solidifying agent.

FORMULA

Gram weight per liter: 25.0gm/L
Soluble Starch 10.0gm
Beef Extract 3.0gm
Agar 12.0gm

Final pH 7.5 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 25.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool to 45-50ºC.

5. Dispense into sterile petri dishes as desired.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. G294.

LIMITATIONS

Since the nutritional requirements of organisms vary, some strains may be encountered that fail to grow or grow poorly on this medium.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Bacillus subtilis
ATCC ® 6633
A 40-48hr 35°C Aerobic Growth; positive starch hydrolysis
Escherichia coli
ATCC ® 25922
A 40-48hr 35°C Aerobic Growth; negative starch hydrolysis
Staphylococcus aureus
ATCC ® 25923
B 40-48hr 35°C Aerobic Growth; negative starch hydrolysis

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Starch Agar powder should appear homogeneous, free-flowing, and light beige in color. The prepared media should appear slightly opalescent without significant precipitate, and light amber in color.

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

4. Vedder. 1915. J. Infect. Dis. ; 16:385.

5. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

6. Atlas, R. 1993. Handbook of Microbiological Media, CRC Press, Boca Raton, FL.


ATCC is a registered trademark of the American Type Culture Collection.

062116gr