CRITERION™ TAT BROTH BASE
|Cat. no. C7030||CRITERION™ TAT Broth Base||56gm|
|Cat. no. C7031||CRITERION™ TAT Broth Base||500gm|
|Cat. no. C7032||CRITERION™ TAT Broth Base||2kg|
|Cat. no. C7033||CRITERION™ TAT Broth Base||10kg|
|Cat. no. C7034||CRITERION™ TAT Broth Base||50kg|
Hardy Diagnostics CRITERION™ TAT Broth Base is used for cultivating microorganisms from highly viscous or gelatinous materials.
This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.
TAT (Trypton-Azolectin-Tween®) Broth is recommended for sterility testing of viscous materials, such as salves or ointments. It is especially adapted to the sterility testing of cosmetics. Cosmetics and pharmaceutical products are subject to contamination during manufacturing and use by consumers.(7) Preservatives are used in aqueous products to make them self-sterilizing for vegetative bacteria, yeasts, and molds.
CRITERION™ TAT Broth Base is an enrichment medium developed to isolate and cultivate microorganisms. CRITERION™ TAT Broth Base conforms to the formula specified by U.S. Pharmacopeia for use in Microbial Limit Tests.(6)
Pancreatic digest of casein provides the nitrogen, vitamins, amino acids, and carbon in CRITERION™ TAT Broth Base. Lecithin (azolectin) neutralizes preservatives in the cosmetics or pharmaceutical products, allowing bacteria to grow.
|Gram weight per liter:||28.0gm/L|
|Pancreatic Digest of Casein||23.0gm|
Final pH 7.2 +/- 0.2 at 25ºC.
* Adjusted and/or supplemented as required to meet performance criteria.
STORAGE AND SHELF LIFE
Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original off-white.
Store the prepared culture media at 2-30ºC.
METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA
1. Suspend 28.0gm of the dehydrated culture media in 960ml of distilled or deionized water.
2. Add 40ml of Tween® 20.
3. Heat to 50-60ºC.
4. Let stand 15-30 minutes with occasional agitation to dissolve completely.
5. Autoclave at 121ºC. for 15 minutes.
6. Dispense as desired into sterile containers.
PROCEDURE AND INTERPRETATION OF RESULTS
For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. K251.
Since the nutritional requirements of organisms vary, some strains may be encountered that fail to grow or grow poorly on this medium.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as autoclaves, incinerators, incubators, and Tween® 20, etc., are not provided.
|Test Organisms||Inoculation Method*||Incubation||Results|
ATCC ® 25922
ATCC ® 27853
ATCC ® 25923
User Quality Control
CRITERION™ TAT Broth Base powder should appear homogeneous, free-flowing, and beige in color. The prepared media should appear clear to slightly opalescent, and light amber in color.
1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.
2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.
3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.
4. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.
5. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.
6. The United States Pharmacopeial Convention. 1995. The United States Pharmacopeia, 23rd ed. Microbial Test Limits, p. 1681-1686. The United States Pharmacopeial Convention, Inc. Rockville, MD.
7. Orth, D.S. 1993. Handbook of cosmetic microbiology. Marcel Dekker, Inc. New York, NY.
ATCC is a registered trademark of the American Type Culture Collection.
Tween is a registered trademark of ICI Americas, Inc.