CRITERION™ TSA BLOOD AGAR BASE

Cat. no. C5220 CRITERION™ TSA Blood Agar Base 77gm
Cat. no. C5221 CRITERION™ TSA Blood Agar Base 500gm
Cat. no. C5222 CRITERION™ TSA Blood Agar Base 2kg
Cat. no. C5223 CRITERION™ TSA Blood Agar Base 10kg
Cat. no. C5224 CRITERION™ TSA Blood Agar Base 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ TSA Blood Agar Base is used with blood for isolation of fastidious microorganisms when hemolytic reactions are important. This medium is also recommended for use in the cultivation, storage, and transportation of pure cultures of bacteria.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

TSA Blood Agar Base is typically supplemented with sheep, rabbit, or horse blood, in various concentrations, to facilitate the growth of some organisms, and for the observation of hemolytic reactions. The absence of reducing sugars and carbohydrates allows the hemolysis to occur without hindrance.

TSA Blood Agar Base contains digests of soybean meal and casein which provides amino acids and other nitrogenous compounds making it a nutritious medium for many microorganisms. Sodium chloride is added to maintain the osmotic equilibrium. This medium may be supplemented with blood to provide a more nutritious medium for fastidious organisms, or with antimicrobials to provide a selective medium for specific organisms out of a mixed flora sample.

FORMULA

Gram weight per liter: 38.5gm/L
Pancreatic Digest of Casein 15.0gm
Peptic Digest of Soybean Meal 5.0gm
Sodium Chloride 5.0gm
Agar 13.5gm

Final pH 7.3 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 38.5gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

For use with blood:

1. Prepare media as above.

2. Cool to 45-50ºC. and aseptically add defibrinated blood with thorough mixing.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. A10.

LIMITATIONS

Neisseria gonorrhoeae may grow on this media with added blood due to the highly enriched nature of the formula.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results**
Time Temperature Atmosphere
Streptococcus pyogenes
ATCC ® 19615
A 18-24hr 35°C Aerobic Growth; beta-hemolysis
Streptococcus pneumoniae
ATCC ® 6305
A 18-24hr 35°C Aerobic Growth; alpha-hemolysis
Staphylococcus aureus
ATCC ® 25923
A 18-24hr 35°C Aerobic Growth; white colonies
Escherichia coli
ATCC ® 25922
A 18-24hr 35°C Aerobic Growth; off-white colonies
Enterococcus faecalis
ATCC ® 29212
A 18-24hr 35°C Aerobic Growth; non-hemolytic

User Quality Control

** Expected results when prepared with the addition of 5% sheep blood.

PHYSICAL APPEARANCE

CRITERION™ Tryptic Soy Blood Agar Base powder should appear homogeneous, free-flowing, and light beige in color. The prepared basal media should appear slightly opalescent, and light amber in color. The prepared media, with the addition of blood, should appear opaque, and red in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Greenberg, A.E., et al. (ed.). 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. APHA, Washington, D.C.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

6. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

7. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

8. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.


ATCC is a registered trademark of the American Type Culture Collection.

062116gr