CRITERION™ TSA (TRYPTIC SOY AGAR) BLOOD BASE, pH 7.4

Cat. no. C7990 CRITERION™ TSA (Tryptic Soy Agar) Blood Base, pH 7.4 80gm
Cat. no. C7991 CRITERION™ TSA (Tryptic Soy Agar) Blood Base, pH 7.4 500gm
Cat. no. C7992 CRITERION™ TSA (Tryptic Soy Agar) Blood Base, pH 7.4 2kg
Cat. no. C7993 CRITERION™ TSA (Tryptic Soy Agar) Blood Base, pH 7.4 10kg
Cat. no. C7994 CRITERION™ TSA (Tryptic Soy Agar) Blood Base, pH 7.4 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ TSA (Tryptic Soy Agar) Blood Base, pH 7.4 is recommended for use as a general purpose growth media for the isolation, cultivation, and differentiation of a wide variety of fastidious microorganisms when hemolytic reactions may be important.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

CRITERION™ TSA Blood Base, pH 7.4 is typically supplemented with either sheep, rabbit, or horse blood, in various concentrations to facilitate the growth of fastidious microorganisms, and for the observation of hemolytic reactions. The absence of reducing sugars and carbohydrates allows hemolysis to occur without hindrance. This medium was created to enhance growth and to improve hemolytic reactions of pathogenic microorganisms

CRITERION™ TSA Blood Agar Base, pH 7.4 is slightly more alkaline than traditional TSA Blood Agar Base. However, this medium contains digests of soybean meal and casein which provide amino acids and other nitrogenous compounds that promote the growth of microorganisms. Likewise, sodium chloride is added to maintain osmotic equilibrium. This medium may be supplemented with blood in various concentrations to provide a more nutritious medium for fastidious microorganisms, or with antimicrobials to provide a selective medium to obtain specific microbes from a mixed flora sample.

FORMULA*

Gram weight per liter: 40.0gm/L
Casein Peptone 15.0gm
Soy Peptone 5.0gm
Sodium Chloride 5.0gm
Agar 15.0gm

Final pH 7.4 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 40.0gm of dehydrated culture media in 1 liter of distilled or deionized water. Stir to mix thoroughly.

2. Heat to boiling to dissolve completely. DO NOT OVERHEAT.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

For use with blood:

1. Prepare media as above.

2. Cool to 45-50ºC. and aseptically add defibrinated blood with thorough mixing.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references.

LIMITATIONS

Neisseria gonorrhoeae may grow on this media with added blood due to the highly enriched nature of the formula.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Staphylococcus aureus
ATCC ® 25923
A 24hr 35°C Aerobic Growth
Escherichia coli
ATCC ® 25922
A 24hr 35°C Aerobic Growth
Streptococcus pyogenes
ATCC ® 19615
A 24hr 35°C Aerobic Growth
Enterococcus faecalis
ATCC ® 29212
A 24hr 35°C Aerobic Growth

User Quality Control

Physical Appearance

CRITERION™ TSA (Tryptic Soy Agar) Blood Base, pH 7.4 powder should appear homogeneous, free-flowing, and light beige in color. The prepared media, without the addition of blood, should appear slightly opalescent and light amber in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Greenberg, A.E., et al. (ed.). 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. APHA, Washington, D.C.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

6. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

7. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

8. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.


ATCC is a registered trademark of the American Type Culture Collection.

062716gr