CRITERION™ TRYPTIC SOY AGAR WITH LECITHIN AND TWEEN® 80

Cat. no. C7130 CRITERION™ Tryptic Soy Agar with Lecithin and Tween® 80 102.2gm
Cat. no. C7131 CRITERION™ Tryptic Soy Agar with Lecithin and Tween® 80 500gm
Cat. no. C7132 CRITERION™ Tryptic Soy Agar with Lecithin and Tween® 80 2kg
Cat. no. C7133 CRITERION™ Tryptic Soy Agar with Lecithin and Tween® 80 10kg
Cat. no. C7134 CRITERION™ Tryptic Soy Agar with Lecithin and Tween® 80 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Tryptic Soy Agar with Lecithin and Tween® 80 is recommended for use in the detection and enumeration of microorganisms in replicate plating techniques. Also used for the detection and enumeration of microorganisms present on the surfaces of sanitary importance.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

The formulation of Tryptic Soy Agar with Lecithin and Tween ® 80 is prepared according to the United States Pharmacopeia (USP) standard formula for Soybean-Casein Digest Agar Medium.(9) It is also included in approved procedures in the Compendium of Methods for the Microbiological Examination of Foods and in Standard Methods for the Examination of Water and Wastewater.(4,8)

Tryptic Soy Agar with Lecithin and Tween® 80 contains digests of soybean meal and casein which provides amino acids and other nitrogenous compounds making it a nutritious medium for many microorganisms. Sodium chloride is added to maintain the osmotic equilibrium. Dextrose is added to serve as an energy source. Quaternary ammonia compounds are neutralized by lecithin while phenolic disinfectants and hexacholorophene are neutralized by Tween® 80. Together, lecithin and Tween® 80 neutralize ethanol. This medium may be supplemented with blood to provide a more nutritious medium for fastidious organisms, or with antimicrobials to provide a selective medium for specific organisms out of a mixed flora sample.

FORMULA

Gram weight per liter: 45.7gm/L
Pancreatic Digest of Casein 15.0gm
Peptic Digest of Soybean Meal 5.0gm
Sodium Chloride 5.0gm
Lecithin 0.7gm
Tween® 80 5.0gm
Agar 15.0gm

Final pH 7.3 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-8ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not homogeneous, moist, and lumpy or if the color has changed from its original light beige.

Store the prepared media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 51.1gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Dispense into sterile petri dishes, or as desired.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. G41.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Bacillus subtilis
ATCC ® 6633
J 1-3 days 35°C Aerobic Growth
Candida albicans
ATCC ® 10231
J 1-3 days 30°C Aerobic Growth
Aspergillus brasiliensis
formerly A. niger
ATCC ® 16404

J 1-3 days 30°C Aerobic Growth

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Tryptic Soy Agar with Lecithin and Tween® 80 powder should appear homogeneous, moist, and lumpy, and beige in color. The prepared media should appear slightly hazy, and light amber in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

6. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

7. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

8. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.

9. U.S. Pharmacopeia, 27nd rev. 2004. U.S. Pharmacopeial Convention, Rockville, MD.


ATCC is a registered trademark of the American Type Culture Collection.
Tween is a registered trademark of ICI Americas, Inc.

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