CRITERION™ TRYPTIC SOY BROTH (TSB)
|Cat. no. C7140||CRITERION™ Tryptic Soy Broth||60gm|
|Cat. no. C7141||CRITERION™ Tryptic Soy Broth||500gm|
|Cat. no. C7142||CRITERION™ Tryptic Soy Broth||2kg|
|Cat. no. C7143||CRITERION™ Tryptic Soy Broth||10kg|
|Cat. no. C7144||CRITERION™ Tryptic Soy Broth||50kg|
Hardy Diagnostics CRITERION™ Tryptic Soy Broth is recommended for use as a general purpose medium for the isolation and cultivation of a wide variety of microorganisms.
This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.
Tryptic Soy Broth is widely used for the cultivation of microorganisms from environmental sources, supporting the growth of the majority of bacteria and fungi. Tubes of this medium may be used for preparing dilutions of organism for colony counts and preparation of standard inocula. Tryptic Soy Broth is also recommended for use in sterility testing for the detection of contamination with low incidence fungi and aerobic bacteria.(1)
Tryptic Soy Broth, also known as Soybean-Casein Digest, conforms to the formula given by the U.S. Pharmacopeia.(1) This medium contains digests of soybean meal and casein, which provide amino acids and other nitrogenous substances, making it a highly nutritious medium for a variety of organisms. Sodium chloride is added to maintain the osmotic equilibrium. Dextrose is incorporated as an energy source. Dipotassium phosphate is included in the formulation as a buffer to maintain the pH. The medium can also be supplemented with Tween® 80 or other non-inhibitory surface-active agent to assist in the suspension of water insoluble substances for the microbiological examination of non-sterile products.(1)
|Gram weight per liter:||30.0gm/L|
|Pancreatic Digest of Casein||17.0gm|
|Papaic Digest of Soybean Meal||3.0gm|
Final pH 7.3 +/- 0.2 at 25ºC.
* Adjusted and/or supplemented as required to meet performance criteria.
STORAGE AND SHELF LIFE
Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.
Store the prepared culture media at 2-30ºC.
METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA
1. Suspend 30.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.
2. Heat to boiling and mix to dissolve completely.
3. If necessary, add 1.0gm/L or appropriate concentration of sterile Tween® 80 (Cat. no. U180) or other noninhibitory surface-active agent to assist in the suspension of poorly wettable substances.(1)
4. Sterilize in the autoclave at 121ºC. for 15 minutes.
PROCEDURE AND INTERPRETATION OF RESULTS
For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. no. K89 or K82 (for USP).
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.
|Test Organisms||Inoculation Method*||Incubation||Results|
ATCC ® 10231
ATCC ® 6633
ATCC ® 25923
ATCC ® 6538
ATCC ® 9027
ATCC ® 25922
ATCC ® 8739
ATCC ® 14028
User Quality Control
CRITERION™ Tryptic Soy Broth powder should appear homogeneous, free-flowing, and light beige in color. The prepared media should appear translucent, and light amber in color.
1. United States Pharmacopoeia and National Formulary (USP-NF). Rockville, MD: United States Pharmacopeial Convention.
2. U.S. Food and Drug Administration. Bacteriological Analytical Manual. AOAC, Arlington, VA.
3. American Public Health Association. Standard Methods for the Examination of Dairy Products, APHA, Washington, D.C.
4. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.
5. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.
6. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.
7. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.
8. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.
ATCC is a registered trademark of the American Type Culture Collection.
Tween is a registered trademark of ICI Americas, Inc.