CRITERION™ TRYPTIC SOY BROTH (TSB)

Cat. no. C7140 CRITERION™ Tryptic Soy Broth 60gm
Cat. no. C7141 CRITERION™ Tryptic Soy Broth 500gm
Cat. no. C7142 CRITERION™ Tryptic Soy Broth 2kg
Cat. no. C7143 CRITERION™ Tryptic Soy Broth 10kg
Cat. no. C7144 CRITERION™ Tryptic Soy Broth 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Tryptic Soy Broth is recommended for use as a general purpose medium for the isolation and cultivation of a wide variety of microorganisms.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Tryptic Soy Broth is widely used for the cultivation of microorganisms from environmental sources, supporting the growth of the majority of bacteria and fungi. Tubes of this medium may be used for preparing dilutions of organism for colony counts and preparation of standard inocula. Tryptic Soy Broth is also recommended for use in sterility testing for the detection of contamination with low incidence fungi and aerobic bacteria.(1)

Tryptic Soy Broth, also known as Soybean-Casein Digest, conforms to the formula given by the U.S. Pharmacopeia.(1) This medium contains digests of soybean meal and casein, which provide amino acids and other nitrogenous substances, making it a highly nutritious medium for a variety of organisms. Sodium chloride is added to maintain the osmotic equilibrium. Dextrose is incorporated as an energy source. Dipotassium phosphate is included in the formulation as a buffer to maintain the pH. The medium can also be supplemented with Tween® 80 or other non-inhibitory surface-active agent to assist in the suspension of water insoluble substances for the microbiological examination of non-sterile products.(1)

FORMULA*

Gram weight per liter: 30.0gm/L
Pancreatic Digest of Casein 17.0gm
Sodium Chloride 5.0gm
Papaic Digest of Soybean Meal 3.0gm
Dextrose 2.5gm
Dipotassium Phosphate 2.5gm

Final pH 7.3 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared culture media at 2-30ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 30.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. If necessary, add 1.0gm/L or appropriate concentration of sterile Tween® 80 (Cat. no. U180) or other noninhibitory surface-active agent to assist in the suspension of poorly wettable substances.(1)

4. Sterilize in the autoclave at 121ºC. for 15 minutes.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. no. K89 or K82 (for USP).

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Candida albicans **
ATCC ® 10231
J 1-3 days 30-35°C Aerobic Growth
Bacillus subtilis **
ATCC ® 6633
J 1-3 days 35°C Aerobic Growth
Staphylococcus aureus
ATCC ® 25923
A 24-48hr 35°C Aerobic Growth
Staphylococcus aureus **
ATCC ® 6538
J 1-3 days 35°C Aerobic Growth
Pseudomonas aeruginosa **
ATCC ® 9027
J 1-3 days 35°C Aerobic Growth
Escherichia coli
ATCC ® 25922
A 24-48hr 35°C Aerobic Growth
Escherichia coli **
ATCC ® 8739
J 1-3 days 35°C Aerobic Growth
Salmonella enterica **
ATCC ® 14028
J 1-3 days 35°C Aerobic Growth

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Tryptic Soy Broth powder should appear homogeneous, free-flowing, and light beige in color. The prepared media should appear translucent, and light amber in color.

REFERENCES

1. United States Pharmacopoeia and National Formulary (USP-NF). Rockville, MD: United States Pharmacopeial Convention.

2. U.S. Food and Drug Administration. Bacteriological Analytical Manual. AOAC, Arlington, VA.
http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006949.htm.

3. American Public Health Association. Standard Methods for the Examination of Dairy Products, APHA, Washington, D.C.

4. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.

5. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.

6. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

7. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

8. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.


ATCC is a registered trademark of the American Type Culture Collection.
Tween is a registered trademark of ICI Americas, Inc.

062716gr