Cat. no. C9140 CRITERION™ Tryptic Soy Broth #2 60gm
Cat. no. C9141 CRITERION™ Tryptic Soy Broth #2 500gm
Cat. no. C9142 CRITERION™ Tryptic Soy Broth #2 2kg
Cat. no. C9143 CRITERION™ Tryptic Soy Broth #2 10kg


Hardy Diagnostics CRITERION™ Tryptic Soy Broth #2 is recommended for use as a general purpose medium for the isolation and cultivation of a wide variety of microorganisms.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.


Tryptic Soy Broth is widely used for the cultivation of microorganisms from environmental sources, supporting the growth of the majority of bacteria and fungi. Tubes of this medium may be used for preparing dilutions of organism for colony counts and preparation of standard inocula. Tryptic Soy Broth is also recommended for use in sterility testing for the detection of contamination with low incidence fungi and aerobic bacteria.(8,9)

Tryptic Soy Broth, also known as Soybean-Casein Digest Medium, conforms to the formula given by the U.S. Pharmacopeia.(8) This medium contains digests of soybean meal and casein, which provide amino acids and other nitrogenous substances, making it a highly nutritious medium for a variety of organisms. Sodium chloride is added to maintain the osmotic equilibrium. Dextrose is incorporated as an energy source. Dipotassium phosphate is included in the formulation as a buffer to maintain pH. The peptones used in Tryptic Soy Broth #2 are district from those used in conventional Tryptic Soy Broth. This is a non-irradiated product and requires sterilization by autoclaving prior to use.


Gram weight per liter: 30.0gm/L
Pancreatic Digest of Casein 17.0gm
Sodium Chloride 5.0gm
Papaic Digest of Soybean Meal 3.0gm
Dextrose 2.5gm
Dipotassium Phosphate 2.5gm

Final pH 7.3 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.


Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original light beige.

Store the prepared culture media at 2-30ºC.



1. Suspend 30.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.


For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. no. K89 or K82 (for USP).(1-9)


This product must be sterilized by autoclaving and not by cold filtration.


Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.


Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Candida albicans **
ATCC ® 10231
J 1-3 days 30-35°C Aerobic Growth
Bacillus subtilis **
ATCC ® 6633
J 1-3 days 35°C Aerobic Growth
Staphylococcus aureus
ATCC ® 25923
A 24-48hr 35°C Aerobic Growth
Staphylococcus aureus **
ATCC ® 6538
J 1-3 days 35°C Aerobic Growth
Pseudomonas aeruginosa **
ATCC ® 9027
J 1-3 days 35°C Aerobic Growth
Escherichia coli
ATCC ® 25922
A 24-48hr 35°C Aerobic Growth
Escherichia coli **
ATCC ® 8739
J 1-3 days 35°C Aerobic Growth
Salmonella enterica **
ATCC ® 14028
J 1-3 days 35°C Aerobic Growth

User Quality Control

** Tested in accordance with USP <61> and <62>.(8,9)


CRITERION™ Tryptic Soy Broth powder should appear homogeneous, free-flowing, and light beige in color. The prepared media should appear translucent, and light amber in color.


1. U.S. Food and Drug Administration. Bacteriological Analytical Manual. AOAC, Arlington, VA.

2. Marshall, R.T., (ed.). 1992. Standard Methods for the Examination of Dairy Products, 16th ed. APHA, Washington, D.C.

3. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.

4. Greenberg, A.E., et al. (ed.). 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. APHA, Washington, D.C.

5. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

6. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

7. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

8. The Official Compendia of Standards. USP General Chapter <61> Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests. USP-NF. United States Pharmacopeial Convention Inc., Rockville, MD.

9. The Official Compendia of Standards. USP General Chapter <62> Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms. USP-NF. United States Pharmacopeial Convention Inc., Rockville, MD.

ATCC is a registered trademark of the American Type Culture Collection.