CRITERION™ THIOGLYCOLLATE MEDIUM WITHOUT INDICATOR

Cat. no. C7080 CRITERION™ Thioglycollate Medium without Indicator 58gm
Cat. no. C7081 CRITERION™ Thioglycollate Medium without Indicator 500gm
Cat. no. C7082 CRITERION™ Thioglycollate Medium without Indicator 2kg
Cat. no. C7083 CRITERION™ Thioglycollate Medium without Indicator 10kg
Cat. no. C7084 CRITERION™ Thioglycollate Medium without Indicator 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ Thioglycollate Medium without Indicator is recommended for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

The addition of a small amount of agar in Thioglycollate Medium without Indicator aids in the initiation and growth of small inocula and anaerobes by impeding the diffusion of oxygen into the medium. It also retards the dispersion of CO2 and the reducing substance from the microenvironment surrounding the inoculum. Sodium thioglycollate is a reducing agent which maintains a low oxygen tension by removing molecular oxygen from the environment. Peroxides, which may be lethal to many anaerobic organisms, are not formed under this condition. Cystine and casein supply carbon and nitrogenous compounds, dextrose is added as another energy source, and sodium chloride maintains osmotic equilibrium.

Certain additives can be incorporated into the Thioglycollate Medium as desired. Yeast extract or papaic digest of soybean meal may be added as growth enhancers. Hemin can be incorporated to supply X factor for the stimulated growth of many fastidious organisms, and vitamin K because it is a growth requirement for some gram-positive spore-formers and Bacteroides species. Calcium carbonate chips may be added to act as a buffer for the medium and prevents buildup of toxic acids.

FORMULA

Gram weight per liter: 29.0gm/L
Pancreatic Digest of Casein 15.0gm
Dextrose 5.0gm
Yeast Extract 5.0gm
Sodium Chloride 2.5gm
Sodium Thioglycollate 0.5gm
L-Cystine 0.25gm
Agar 0.75gm

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original beige.

Store the prepared culture media at 2-30ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 29.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat to boiling and mix to dissolve completely.

3. Dispense as desired into autoclavable containers.

4. Sterilize in the autoclave at 121ºC. for 15 minutes.

5. Tighten lids immediately after autoclaving (while still warm) to reduce oxidation.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. K29.

LIMITATIONS

In test samples, the proper surface to volume ratio of the Thioglycollate Medium must be maintained to avoid oxidation of the medium, making it unsuitable for microaerophilic and anaerobic growth.

A slight turbidity or haziness may be present due to the small amount of agar in the medium. When the media has been boiled it appears clear.

Do not boil media more than once, as frequent boiling may lead to toxic products forming in the medium. If it is suspected that the medium has more than 30% oxidation after boiling, it should be discarded.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Clostridium novyi A
ATCC ® 7659
A 24-48hr 35°C Aerobic Growth
Staphylococcus aureus
ATCC ® 25923
A 24-48hr 35°C Aerobic Growth
Candida albicans
ATCC ® 10231
A 24-48hr 35°C Aerobic Growth
Bacillus subtilis
ATCC ® 6633
A 24-48hr 35°C Aerobic Growth

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Thioglycollate Medium without Indicator powder should appear homogeneous, free-flowing, and beige in color. The prepared media should appear translucent, and amber in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.


ATCC is a registered trademark of the American Type Culture Collection.

062716gr