CRITERION™ TRYPTOSE PHOSPHATE BROTH

Cat. no. C7210 CRITERION™ Tryptose Phosphate Broth 59gm
Cat. no. C7211 CRITERION™ Tryptose Phosphate Broth 500gm
Cat. no. C7212 CRITERION™ Tryptose Phosphate Broth 2kg
Cat. no. C7213 CRITERION™ Tryptose Phosphate Broth 10kg

INTENDED USE

Hardy Diagnostics CRITERION™ Tryptose Phosphate Broth is recommended for use as a general purpose growth media for the isolation, cultivation, and differentiation of a wide variety of fastidious microorganisms.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

CRITERION™ Tryptose Phosphate Broth is an infusion-free buffered medium recommended for the cultivation of fastidious, pathogenic microorganisms. Tryptose Phosphate Broth is valuable in tissue culture procedures, as shown by Ginsberg, Gold, and Jordan. The peptone content of Tryptose Phosphate Broth is considered to be a stimulating factor for cells. Tryptose Phosphate Broth is specified for cell culture procedures.(1,2)

Nitrogen, vitamins, and carbon sources are provided by peptones. Dextrose in the carbohydrate source. Sodium chloride maintains the osmotic environment and disodium phosphate is the buffering agent.

The addition of 0.1-0.2% agar to Tryptose Phosphate Broth facilitates anaerobic growth, and aids in dispersion of reducing substances and CO2 formed in the environment.(3) The low agar concentration provides suitable conditions for aerobic growth in the upper zone, and microaerophilic and anaerobic growth in the lower zone.

FORMULA*

Gram weight per liter: 29.5gm/L
Pancreatic Digest of Casein 10.0gm
Peptic Digest of Animal Tissue 10.0gm
Sodium Chloride 5.0gm
Disodium Phosphate 2.5gm
Dextrose 2.0gm

Final pH 7.3 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original beige.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 29.5gm of the dehydrated culture media in one liter of distilled or deionized water. Stir to mix thoroughly.

2. Heat as necessary to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool to 45-50ºC.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Neisseria meningitidis
ATCC ® 13090
A 18-48hr 35°C Aerobic Growth
Staphylococcus epidermidis
ATCC ® 12228
A 18-48hr 35°C Aerobic Growth
Streptococcus pneumoniae
ATCC ® 6305
A 18-48hr 35°C Aerobic Growth
Streptococcus pyogenes
ATCC ® 19615
A 18-48hr 35°C Aerobic Growth

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ Tryptose Phosphate Broth powder should appear homogeneous, free-flowing, and beige in color. The prepared media should appear clear and golden in color.

REFERENCES

1. Ginsberg, Gold, and Jordan. 1955. Proc. Soc. Exp. Bio. Med.; 89:66.

2. U.S. Food and Drug Administration. Bacteriological Analytical Manual. AOAC, Arlington, VA.
http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006949.htm.

3. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

4. Vanderzant, C. and D. R. Splittstoesser, (ed). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.


ATCC is a registered trademark of the American Type Culture Collection.

062716gr