CRITERION™ UVM MODIFIED LISTERIA ENRICHMENT BROTH

Cat. no. C7240 CRITERION™ UVM Modified Listeria Enrichment Broth 104gm
Cat. no. C7241 CRITERION™ UVM Modified Listeria Enrichment Broth 500gm
Cat. no. C7242 CRITERION™ UVM Modified Listeria Enrichment Broth 2kg
Cat. no. C7243 CRITERION™ UVM Modified Listeria Enrichment Broth 10kg
Cat. no. C7244 CRITERION™ UVM Modified Listeria Enrichment Broth 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ UVM Modified Listeria Enrichment Broth is recommended for the rapid isolation of Listeria monocytogenes.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

Listeria monocytogenes is a widespread problem in public health and food industries. Listeria monocytogenes was first described in 1926 by Murray, Webb and Swann, and can cause human illness and death, particularly in immunocompromised individuals and pregnant women.(1,2) The first reported food-borne outbreak of listeriosis was in 1985.(3) The principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes.(4)

This organism has been isolated from turkey frankfurters, coleslaw, pasteurized milk, Mexican-style cheese, pate, and pickled pork tongue.(5) Listeria monocytogenes is present in a wide range of unprocessed foods as well as in soil, sewage, silage, and river water.(6) Listeria species can grow over a pH range of 5.0-9.6 and survive in food products with pH levels outside of that range.(7)

UVM Modified Listeria Enrichment Broth is a modification of the formula described by Donnelly and Baigent.(9) It is used for selective enrichment of Listeria spp. from food and clinical specimens.

CRITERION™ UVM Modified Listeria Enrichment Broth contains beef extract, yeast extract, pancreatic digest of casein, and peptic digest of animal tissue which provide nitrogen, vitamins and minerals. Sodium chloride is added to maintain osmotic balance and phosphate is added as a buffer. Nalidixic acid inhibits gram-negative organisms and acriflavine hydrochloride inhibits many gram-positive bacteria. Esculin is hydrolyzed by Listeria species.

FORMULA

Gram weight per liter: 52.0gm/L
Sodium Chloride 20.0gm
Disodium Phosphate 9.6gm
Pancreatic Digest of Casein 5.0gm
Peptic Digest of Animal Tissue 5.0gm
Beef Extract 5.0gm
Yeast Extract 5.0gm
Monopotassium Phosphate 1.35gm
Esculin 1.0gm
Nalidixic Acid 20.0mg
Acriflavine HCl 12.0mg

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original beige.

Store the prepared culture media at 2-30ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 52.0gm of the dehydrated culture media in 1 liter of distilled or deionized water.

2. Heat as necessary to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool to 45-50ºC.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. K96.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Listeria monocytogenes
ATCC ® 19114
A 18-48hr 35°C Aerobic Growth
Staphylococcus aureus
ATCC ® 29523
B 18-48hr 35°C Aerobic Partial to complete inhibition
Escherichia coli
ATCC ® 25922
B 18-48hr 35°C Aerobic Inhibited
Enterococcus faecalis
ATCC ® 29212
B 18-48hr 35°C Aerobic Inhibited

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ UVM Modified Listeria Enrichment Broth powder should appear homogeneous, free-flowing, and beige in color. The prepared media should appear very slightly opalescent with a fine precipitate, and amber in color.

REFERENCES

1. Murray, E.G.D., R.A. Webb, and M.B.R. Swann. 1926. A disease of rabbits characterized by large mononuclear leucocytosis caused by a hitherto undescribed bacillus Bacterium monocytogenes. J. Path. Bact.; 29:407-0439.

2. Monk, J.D., R.S. Clavero, L.R. Beuchat, M.P. Doyle and R.E. Brackett. 1994. Irradiation inactivation of Listeria monocytogenes and Staphylococcus aureus in low- and high-fat, frozen and refrigerated ground beef. J. Food Prot.; 57:969-974.

3. Wehr, H.M. 1987. Listeria monocytogenes - a current dilemma special report. J. Assoc. Off. Anal. Chem.; 70:769-772.

4. Bremer, P.J., and C.M. Osborne. 1995. Thermal-death times of Listeria monocytogenes in green shell mussels (Perna canaliculus) prepared for hot smoking. J. Food Prot.; 58:604-608.

5. Grau, F.H., and P.B. Vanderlinde. 1992. Occurrence, numbers, and growth of Listeria monocytogenes on some vacuum-packaged processed meats. J. Food Prot.; 55:4-7.

6. Patel, J.R., C.A. Hwang, L.R. Beuchat, M.P. Doyle, and R.E. Brackett. 1995. Comparison of oxygen scavengers for their ability to enhance resuscitation of heat-injured Listeria monocytogenes. J. Food Prot.; 58:244-250.

7. Donnelly, C.W., R.E. Brackett, D. Doores, W.H. Lee, and J. Lovett. 1992. Compendium of methods for the microbiological examination of foods, 3rd ed. American Public Health Association, Washington, D.C.

8. Kramer, P.A., and D. Jones. 1969. Media selective for Listeria monocytogenes. J. Appl. Bacteriol.; 32:381-394.

9. Donnelly, C.W. and G.J. Baigent. 1986. Method for flow cytometric detection of Listeria monocytogenes in milk. Appl. Environ. Microbiol.; 52:689-695.

10. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

11. Lee, W.H., and D. McClain. 1989. Laboratory Communication No.57. U.S.D.A., F.S.I.S. Microbiology Division, Bethesda, MD.

12. Hayes, P.S., L.M. Graves, B. Swaminathan, G.W. Ajello, G. B. Marcolm, R.E. Weaver, R. Ransom, K. Deaver, B.D. Plikaytis, A. Schuchat, J.D. Wenger, R.W. Pinner, C.V. Broome, and The Listeria Study Group. 1992. Comparison of three selective enrichment methods for the isolation of Listeria monocytogenes from naturally contaminated foods. J. Food Prot.; 55:952-959.


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062716gr