CRITERION™ m Brilliant Green Broth

Cat. no. C7940 CRITERION™ m Brilliant Green Broth 152gm
Cat. no. C7941 CRITERION™ m Brilliant Green Broth 500gm
Cat. no. C7942 CRITERION™ m Brilliant Green Broth 2kg
Cat. no. C7943 CRITERION™ m Brilliant Green Broth 10kg
Cat. no. C7944 CRITERION™ m Brilliant Green Broth 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ m Brilliant Green Broth is for the selective and differential isolation of Salmonella spp. from water by membrane filtration.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

The Salmonella species are responsible for multiple types of clinical infection including gastroenteritis, bacteremia or septicemia, enteric fever, and a carrier state where a formerly infected person can excrete the bacteria up to one year following the withdrawal of symptoms.(1,2) These organisms are common in the environment, though much less frequent than coliforms, and small concentrations can be found in most surface waters.(3) This may require testing of larger amounts of water and the use of multiple enrichment and isolation medias in order to recover all the Salmonella serotypes.(3)

Kristensen et al., in 1925, first described use of Brilliant Green Agar as a primary plating medium for the isolation of Salmonella spp.(4) The original formula was altered by Kauffmann ten years later.(5) Hardy Diagnostics CRITERION™ m Brilliant Green Broth is a modification of Kauffmann's Brilliant Green Agar in which the agar has been removed and the remaining ingredients have been increased.

The current formulation incorporates phenol red as the pH indicator of carbohydrate fermentation and brilliant green as an inhibitory agent that acts against gram-positive organisms and gram-negative bacilli. Peptones provide a source of carbon, nitrogen, vitamins and minerals. Yeast extract supplies complex B vitamins, trace elements and amino acids that help stimulate bacterial growth. The carbohydrates available for bacterial growth are lactose and sucrose while sodium chloride is added to ensure proper osmotic pressure.

FORMULA*

Gram weight per liter: 76.0gm/L
Proteose Peptone No. 3 20.0gm
Lactose 20.0gm
Sucrose 20.0gm
Sodium Chloride 10.0gm
Yeast Extract 6.0gm
Phenol Red 0.16gm
Brilliant Green 0.025gm

Final pH 6.9 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original pink.

Store the prepared culture media at 2-8ºC.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 76gm of the dehydrated culture media in 1 liter of distilled or deionized water. Stir to mix thoroughly.

2. Heat to boiling while stirring to dissolve completely.

3. DO NOT AUTOCLAVE.

4. Cool to room temperature. Dispense 2ml aliquots onto sterile absorbent pads.

5. Use prepared media within twenty-four hours.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as membrane filters, absorbent pads, forceps, swabs, loops, autoclaves, incinerators, incubators, serological and biochemical reagents, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Salmonella enteritidis
ATCC ® 13076
MF 18-24hr 35°C Aerobic Growth; pink to red colonies
Salmonella enterica
ATCC ® 14028
MF 18-24hr 35°C Aerobic Growth; pink to red colonies
Escherichia coli
ATCC ® 25922
MF 18-24hr 35°C Aerobic Partial inhibition; yellow colonies

User Quality Control

Physical Appearance

CRITERION™ m Brilliant Green Broth powder should appear homogeneous, free-flowing, and pink in color. The prepared media should appear slightly opalescent, and greenish-red in color.

REFERENCES

1. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.

4. Kristensen, M., Lester, V. and Jurgens, A. (1925) Use of trypsinised casein, brom-thymol blue, brom-cresol purple, and brilliant green for bacteriological nutrient media. British Journal of Experimental Pathology; 6:291-299.

5. Kauffman, F. 1935. Weitere Erfahrungen mit den kombiniereten Anreicherungsverfahren fur Salmonella bazillen. Zeitschrift fur Hygiene und Infektionskrankheit; 117:26-32.

6. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

7. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

8. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.


ATCC is a registered trademark of the American Type Culture Collection.

062916gr