CRITERION™ m ENDO BROTH

Cat. no. C7550 CRITERION™ m Endo Broth 96gm
Cat. no. C7551 CRITERION™ m Endo Broth 500gm
Cat. no. C7552 CRITERION™ m Endo Broth 2kg
Cat. no. C7553 CRITERION™ m Endo Broth 10kg
Cat. no. C7554 CRITERION™ m Endo Broth 50kg

INTENDED USE

Hardy Diagnostics CRITERION™ m Endo Broth is recommended for the enumeration of coliforms in water by membrane filtration.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

CRITERION™ m Endo Broth follows the formula of the Millipore Filter Corporation.(1) It is used for selectively isolating coliforms from water and other specimens using the membrane filtration technique. m Endo Broth is specified by the American Public Health Association (APHA) for the standard total coliform membrane filtration procedure for testing water and bottled water.(2,3) APHA also specifies using m Endo Broth in the delayed-incubation total coliform procedure by adding sodium benzoate to make m Endo preservative medium.(2) The U.S. Environmental Protection Agency specifies using m Endo Broth in the total coliform water testing methods using single step, two-step, and delayed incubation membrane filtration methods.(4,5)

The medium is composed of yeast extract, pancreatic digest of casein, and peptic digest of animal tissue which supply necessary growth nutrients. Lactose is added as a source of fermentable carbohydrate. The phosphates are included as buffers. Sodium chloride is added to maintain osmotic balance. Sodium deoxycholate and sodium lauryl sulfate are added as inhibitors. Basic fuchsin in the pH indicator and sodium sulfite is added to decolorize the basic fuchsin solution.

FORMULA

Gram weight per liter: 48.0gm/L
Lactose 12.5gm
Pancreatic Digest of Casein 10.0gm
Peptic Digest of Animal Tissue 10.0gm
Sodium Chloride 5.0gm
Dipotassium Phosphate 4.375gm
Sodium Sulfite 2.1gm
Yeast Extract 1.5gm
Monopotassium Phosphate 1.375gm
Basic Fuchsin 1.05gm
Sodium Deoxycholate 0.1gm
Sodium Lauryl Sulfate 0.05gm

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed bottle(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep lid tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original pinkish-purple.

Store prepared media at 2-8ºC. in the dark.

PRECAUTIONS

METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend 48.0gm of the dehydrated culture media in 1 liter of distilled or deionized water containing 20ml of ethanol (95%, not denatured).

2. Heat to boiling and mix to dissolve completely. Do not autoclave.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Escherichia coli
ATCC ® 25922
A 24hr 35°C Aerobic Growth
Salmonella enterica
ATCC ® 14028
A 24hr 35°C Aerobic Growth; colorless
Staphylococcus aureus
ATCC ® 25923
B 24hr 35°C Aerobic Inhibited

User Quality Control

PHYSICAL APPEARANCE

CRITERION™ m Endo Broth powder should appear homogeneous, free-flowing, and pinkish-purple in color. The prepared media should appear opalescent with precipitate, and pale pink in color.

REFERENCES

1. Fifield, C. W. and C.P. Schaufus. 1958. Improved membrane filter medium for the detection of coliform organisms. J. Amer. Water Works Assoc.; 50:193.

2. Eaton, A.D., L.S. Clesceri and A.E. Greenberg (ed.). 1995. Standard Methods for the Examination of Water and Wastewater, 19th ed. American Public Health Association, Washington, D.C.

3. Cowman, S. and R. Kelsey. Bottled water, p. 1031-1036. In C. Canderzant and D.F. Splittstoesser (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. American Public Health Association, Washington, D.C.

4. Bordner, R. and J. Winter (ed.). 1978. Microbiological methods for monitoring the environment, water and wastes. EPA-600/8-78-017. Environmental Monitoring and Support Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Cincinnati, OH.

5. Environmental Protection Agency. 1992. Manual for the certification of laboratories analyzing drinking water. EPA-814B-92-002. Office of Ground Water and Technical Support Division, U.S. Environmental Protection Agency, Cincinnati, OH.


ATCC is a registered trademark of the American Type Culture Collection.

062916gr